Improved Stabilities of Lipase by Immobilization on Eupergit C

Bin Wang; Chun Yong Zhang; Xiu Ying Hu; De Ma; Li Su; Quan Fa Zhou

doi:10.4028/www.scientific.net/AMR.1096.219

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1096Improved Stabilities of Lipase by Immobilization...

Improved Stabilities of Lipase by Immobilization on Eupergit C

Abstract:

Lipase is frequently used for catalyze wide non-natural substrates in order to obtain enantio- and regioselectivie substrates. This study evaluates the stabilities of lipase by immobilization on Eupergit C. The immobilized lipase had improved stability at 60^oC for 60 h compared to the free lipase 1 h at 60^oC. Immobilization resulted in an increase in pH stability over a range of 7.0 - 9.0 and about 54 days for half-life of storage at 4 ^oC. The tolerance of lipase to organic solvents was also improved by immobilization, and the immobilized lipase showed activating activity when exposed to hydrophobic solvents.

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Periodical:

Advanced Materials Research (Volume 1096)

Pages:

219-223

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1096.219

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Online since:

April 2015

Authors:

Bin Wang*, Chun Yong Zhang, Xiu Ying Hu, De Ma, Li Su, Quan Fa Zhou

Keywords:

Eupergit C, Immobilization, Lipase

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* - Corresponding Author

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