Synthesis and Characterization of PVA-Ag Doped Metals (Co, Al) Nanocomposite Thin Films Biosensors for Detection of E. coli in Water

Abstract:

Polyvinyl alcohol (PVA) doped with Ag thin films were synthesized from aqueous solution via sol gel method. The nanoparticle of silver was synthesized by chemical reduction using hydrazine hydrate. PVA-Ag thin films were deposited on the glass substrate by spin coating technique. Samples were varied with different combinations of metals such as Ag-Co and Ag-Al. The solutions and the films were characterized by using XRD, UV-Visible spectroscopy, AFM and TEM. XRD analysis indicates the formation of the single crystal Ag, Co and Al nanoparticles laid on (111) lattice planes. The crystallite sizes decrease when Co and Al are added to the PVA-Ag. UV-Vis absorption spectra confirmed the formation of Ag nanoparticles in the PVA matrix and the resonance plasmon band located at 417, 421 and 429 nm. Surface roughness of PVA-Ag nanocomposite thin film increased with the addition of Co and Al. TEM images show the non-agglomerated spherical particles in all samples. The performance of the sensor has been fabricated using I-V measurement with and without incubated the sensor electrode into E. coli. The result shows PVA-Ag nanocomposite thin film performed the higher sensitivity.

• Periodical: Advanced Materials Research (Volume 1107)
• Pages: 706-711
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1107.706
• Online since: June 2015
• Authors: Norshafadzila Mohammad Naim, Huda Abdullah*, Noor Azwen Noor Azmy, Aidil Abdul Hamid, Mohd Ambar Yarmo, Akrajas Ali Umar, Sahbudin Shaari, Wan Hanna Melini Wan Mokhtar, Zurina Osman
• Keywords: Biosensor, Polyvinyl Alcohol, Silver Nanoparticle, Thin Film

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