Effect of Carbon and Nitrogen Ratio, Mineral Solution & Inoculum Size in the Production of Xylanase Using Oil Palm Leaf

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The production of xylanase by Aspergillus niger ATCC 16404 via solid state fermentation (SSF) system using oil palm leaves (OPL) as substrate was investigated. Fermentation parameters studied using one factor at a time (OFAT) technique, were carbon-nitrogen (C/N) ratio, mineral solution size and inoculums size. It was found that the optimum C/N ratio was at 0.4 with xylanase activity at 16.046 U/min. Meanwhile, the optimum size for both mineral solution size and inoculum size were at 1 ml with the xylanase activity recorded at 14.500 U/min and 19.057 U/min respectively. This shows that that the utilization of OPL as substrates in xylanase production using Aspergillus niger ATCC 16404 was a successful.

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273-278

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July 2015

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© 2015 Trans Tech Publications Ltd. All Rights Reserved

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