Microelectrode Design for Particle Trapping on Bioanalysis Platform

Abstract:

Microelectrode geometry has significant influence on particles trapping techniques used on bioanalysis platforms. In this paper, the particle trapping patterns of dipole, quadrupole and octupole microelectrode using dielectrophoretic force (DEP) are discussed. The microelectrodes were constructed on a metal-insulator-metal platform, built on a silicon nitride (Si₃N₄) coated silicon substrate. The back contact is made from 20 nm nickel-chromium (NiCr) and 100 nm gold (Au) as the first layer. Then, SU-8-2005 (negative photoresist) is used on the second layer to create microcavities for trapping the particles. The third layer, where the three geometries were patterned, is made from 20 nm NiCr and 100 nm Au layers. Prior to fabrication, the particles trapping patterns of the microelectrodes were profiled using a finite element software, COMSOL 3.5a. Trapping patterns for the three geometries were evaluated using polystyrene latex microbeads. Results from the experiment validate simulation studies in term of microelectrode trapping ability up to single particle efficiency. It provides the potential of converting the trapping platform into a lab-on-chip system.

• Periodical: Advanced Materials Research (Volume 1115)
• Pages: 543-548
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1115.543
• Online since: July 2015
• Authors: Siti Noorjannah Ibrahim*, Maan M. Alkaisi
• Keywords: Bio-Analysis Platform, Dielectrophoresis, Microelectrode Design, Particle Trapping

References

[1] M.R. Tomkins, A.W. Jeffery and A. Docoslis: The Canadian Journal of Chemical Engineering. Vol. 86 (2008), p.609.

[2] L. F. Hartley, K.V.I. S Kaler and R. Paul : Journal of Electrostatics. Vol. 46(1999), p.233.

[3] M. Hywel, A.G. Izquiero, D. Bakewell, N.G. Green and A. Ramos: Journal of Physics D: Applied.

[4] E. G. Cen,C. Dalton, Y. Li, S. Adamia, L.M. Pilarski and K.V.I. S Kaler: Journal of Microbiological Methods. Vol. 58(2004), p.387.

[5] L. -C. Hsiung, et al. : Biosensors and Bioelectronics. Vol. 24(2008), p.869.

[6] L. Qian, M. Scott, K.V.I.S. Kaler and R. Paul: Journal of Electrostatics. Vol. 55(2002), p.65.

[7] H. Sorribas, C. Padeste and L. Tiefenauer : Biomaterials. Vol. 23(2002), p.893.

[8] T. Kawabata and M. Washizu: IEEE Transactions on Industry Applications. Vol. 37(2001), p.1625.

[9] S. P. Forry, D.R. Reyes, M. Gaitan, and L. E. Locascio: J. Am. Chem. Soc. Vol. 128(2006), p.13678.

[10] E. Martinez, E. Engel, J.A. Planell and J. Samitier : Annals of Anatomy-Anatomischer Anzeiger. Vol 191(2009), p.126.

DOI: 10.1016/j.aanat.2008.05.006

[11] R.S. Thomas, H. Morgan and N.G. Green: Lab on Chip. Vol. 9 (2009), p.1534.

[12] S. N. Ibrahim, L. Murray, J. J Evans and M.M. Alkaisi: Material Science Forum . Vol. 700 (2011), p.188.

[13] H. A. Pohl : Dielectrophoresis: the behavior of neutral matter in nonuniform electric fields (Cambridge University Press, Cambridge 1978).