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Sensitive Determination for Papain Conjugated CdSe Quantum Dots by Dynamic Light Scattering Analysis
Abstract:
A strong, simple and rapid method for determination enzyme (papain) conjugated quantum dots (QDs) by dynamic light scattering (DLS) is proposed in this report. Yellow CdSe QDs are synthesized with changing the precursor ratio and confirmed by absorption and emission spectra. Shape and size of synthesized QDs are checked by transmission electron microscope (TEM). Both absorption and emission spectra reveal very strong quantum confinement effect as expected; yellow excitonic emission is observed at room temperature for photoluminescence spectra (PL). The peak maxima are appreciably red-shifted when QDs are conjugated with positively charged papain enzyme. The details possible mechanism is described here, which is very interesting and scarcely addressed before.
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19-23
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Online since:
July 2015
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© 2015 Trans Tech Publications Ltd. All Rights Reserved
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