Sensitive Determination for Papain Conjugated CdSe Quantum Dots by Dynamic Light Scattering Analysis

Article Preview

Abstract:

A strong, simple and rapid method for determination enzyme (papain) conjugated quantum dots (QDs) by dynamic light scattering (DLS) is proposed in this report. Yellow CdSe QDs are synthesized with changing the precursor ratio and confirmed by absorption and emission spectra. Shape and size of synthesized QDs are checked by transmission electron microscope (TEM). Both absorption and emission spectra reveal very strong quantum confinement effect as expected; yellow excitonic emission is observed at room temperature for photoluminescence spectra (PL). The peak maxima are appreciably red-shifted when QDs are conjugated with positively charged papain enzyme. The details possible mechanism is described here, which is very interesting and scarcely addressed before.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

19-23

Citation:

Online since:

July 2015

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2015 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] X. Wang, X. Ren, K. Kahen, M. A. Hahn, M. Rajeswaran, S. Maccagnano-Zacher, J. Silcox, G. E. Cragg, A. L. Efros and T. D. Krauss: Nature Vol. 459 (2009), p.686.

DOI: 10.1038/nature08072

Google Scholar

[2] U. Resch-Genger, M. Grabolle, S. Cavaliere-Jaricot, R. Nitschke and T. Nann: Nature Methods Vol. 5 (2008), p.763.

DOI: 10.1038/nmeth.1248

Google Scholar

[3] N. Hildebrandt: ACS Nano Vol. 5(7) (2011), p.5286.

Google Scholar

[4] B.J. Nehilla, T.Q. Vu and T.A. Desai: J. Phys. Chem. B Vol. 109 (44) (2005), p.20724.

Google Scholar

[5] X. Ji, J. Zheng, J. Xu, V.K. Rastogi, T. Cheng, J.J. DeFrank and R.M. Leblanc: J. Phys. Chem. B Vol. 109 (9) (2005), p.3793.

Google Scholar

[6] S. Y. Ding, M. Jones, M.P. Tucker, J.M. Nedeljkovic, J. Wall, M.N. Simon, G. Rumbles and M.E. Himmel: Nano Letters Vol. 3 (2003), p.1581.

DOI: 10.1021/nl034578t

Google Scholar

[7] S. Huang, Q. Xiao, W. Su, P. Li, J. Ma and Z. He: Colloids and surfaces B: Biointerfaces Vol. 102 (2013), p.146.

Google Scholar

[8] B.L. Lpe, A. Shukla, H. Lu, B. Zou, H. Rehage and C.M. Niemeyer: Chem. Phys. Chem. Vol. 7 (2006), p.1112.

Google Scholar

[9] J. Zhang, X. Zhang and J. Y. Zhang: J. Phys. Chem. C Vol. 113(22) (2009), pp.9512-9515.

Google Scholar

[10] W.C.W. Chan, S. Nie: Science Vol. 281 (1998), p. (2016).

Google Scholar

[11] G. Mie: Ann Physik. Vol. 4 (1908), p.377.

Google Scholar

[12] E. Rabani, B. Hetényi, B.J. Berne and L. E. Brus: J. Chem. Phys. Vol. 110 (1999), p.5355.

Google Scholar