In Vitro Release Characteristics of Hydrophobic Breast Cancer Drug Loaded Poly Lactic-co-Glycolic Acid (PLGA) Nanoparticles

Abstract:

The present work focuses on the development of biodegradable PLGA nanoparticles (NPs) for controlled release of a breast cancer drug, letrozole. NPs of different drug-polymer ratio formulations (F1, F2, F3, F4) were fabricated using solvent evaporation technique. Physico-chemical characteristics of these NPs were assessed using dynamic light scattering (DLS) spectrophotometer. In-vitro drug release study was carried out over an extended period of 30 days at 37 °C in simulated physiological fluid. To evaluate the release kinetics, data was fitted to different models. NPs with various sizes and size distributions were obtained by altering the drug-polymer ratio. Zeta potential of PLGA and drug loaded NPs were found to be-29.4± 1.3 mV and-21.0±0.6 mV, respectively. The release kinetics of the drug from NPs was in good agreement with Korsmeyer-Peppas model, ensuring controlled release of the drug from the NPs. In-vitro release studies showed high correlation coefficient (R² = 0.90) for formulation F2 and F3 up to 30 days. It is concluded that NPs with F2 and F3 formulations provide a controlled release of the incorporated drug and, therefore, hold promise to be investigated further in detail.

• Periodical: Advanced Materials Research (Volume 1123)
• Pages: 312-315
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1123.312
• Online since: August 2015
• Authors: Akhtar Jahan Siddiqa*, Koel Chaudhury, Basudam Adhikari
• Keywords: Breast Cancer Drug, Encapsulation, In Vitro, Nanoparticle, PLGA

References

[1] D.B. Shenoy, M.M. Amiji, Poly(ethyleneoxide)-modified poly(epsilon-caprolactone) nanoparticles for targeted delivery of tamoxifen in breast cancer, Int. J. Pharm. 293 (2005) 261-270.

DOI: 10.1016/j.ijpharm.2004.12.010

[2] Information on http: /www. nanomarkets. com.

[3] R.H. Muller, C. M. Keck, Challenges and solutions for the delivery of biotech drugs–a review of drug nanocrystal technology and lipid nanoparticles, J. Biotechnol. 113 (2004) 151-170.

DOI: 10.1016/j.jbiotec.2004.06.007

[4] A. Kumari, S.K. Yadav, S.C. Yadav, Biodegradable polymeric nanoparticles based drug delivery systems, Colloid. Surface B 75 (2010) 1-18.

DOI: 10.1016/j.colsurfb.2009.09.001

[5] X.S. Wu, Synthesis and properties of biodegradable lactic/glycolic acid polymers. Encyclopedic handbook of biomaterials and bioengineering, Marcel Dekker, New York, 2 (1995) 1015-1054.

[6] X.S. Wu, Preparation, characterization, and drug delivery applications of microspheres based on biodegradable lactic/glycolic acid polymers. Encyclopedic handbook of biomaterials and bioengineering. Marcel Dekker, New York, (1995) 1151-1160.

[7] M.J. Schaefer, J. Singh, Effect of tricaprin on the physical characteristics and in vitro release of etoposide from PLGA microspheres, Biomaterials 23 (2002) 3465-3471.

DOI: 10.1016/s0142-9612(02)00053-4

[8] J.M. Anderson, M. S. Shive, Biodegradation and biocompatibility of PLA and PLGA microspheres, Adv. Drug Deliv. Rev. 64 (2012) 72-82.

DOI: 10.1016/j.addr.2012.09.004

[9] X. Liu, K. Nakamura, A.M. Lowman, Composite hydrogels for sustained release of therapeutic agents, Soft. Mat. 1 (2003) 393-408.

DOI: 10.1081/smts-120027080

[10] A.J. Siddiqa, K. Chaudhury, B. Adhikari, Letrozole dispersed on poly (vinyl alcohol) anchored maleic anhydride grafted low density polyethylene: A controlled drug delivery system for treatment of breast cancer, Colloid. Surface B 116 (2014).

DOI: 10.1016/j.colsurfb.2013.12.040

[11] S. Pechenov, B. Shenoy, M. X. Yang, S. K. Basu, A. L. Margolin, Injectable controlled release formulations incorporating protein crystals. J. Controlled Release, 96 (2004) 149-158.

DOI: 10.1016/j.jconrel.2004.01.019

[12] J.W. Vanderhoff, M.S. Elaasser and Ugelstad, J. Polymer emulsification process. U. S. Patent, 4, 177, 177. (1979).