This study discusses the hydrogen-producing properties of continuous stirred tank reactor (CSTR) after inoculating the Biohydrogenbacterium R3. Biohydrogenbacterium R3 grow well and produce H2 stably at temperature of (35±1)°C and HRT of 6 hours. In this study, the optimum range of pH is 4.5~4.6. The amount of biogas production and H2 content maintained at 3.4 L and 47%, respectively. The main liquid end products are ethanol and acetic acid. Dry cell weight changes showed wavy. COD removal is about 16%～18%.