Screening and Identification of a PCBs-Degrading Halophilic Bacterium

Article Preview

Abstract:

Polychlorinated biphenyls (PCBs) are Persistent Organic Pollutants (POPs). This experiment screened a halophilic bacterium from the deep sea sediments, which could grow at the salinity of 15%, and designated as B2.6. The cells of this strain were rod shaped, Gram negative, motile. Growth of this bacterium in the presence of PCBs, as the sole source of carbon and energy, and the experiment indicated that at 30°C when pH value is between 7 and 8, inoculation amount is 5mL, the concentration of PCBs is below 3mg•L-1, and the ratio of degradation could reach 90% at 72h. The traditional morphology, physiological and biochemical properties as well as identified system of Biolog were applied to the bacterial classification. The pattern of PCBs-degrading enzyme production of strain B2.6 was continuous synthesis.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 113-116)

Pages:

2177-2180

Citation:

Online since:

June 2010

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2010 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] CHEN Manrong, YU Lizhong, XU Shiyuang et al. Research of PCBs Pollution in Tidal Flat Sediments of Yangtze River Estuary[J]. Environmental Science and Technology, Issue 5, 2004: 24-28.

Google Scholar

[2] Josephine Borja, Donna Marie Taleon. Polychlorinated biphenyls and their biodegradation[J]. Process Biochemistry, Volume 40, Issue 6, May 2005: 1999-(2013).

DOI: 10.1016/j.procbio.2004.08.006

Google Scholar

[3] Alexander M. Biodegradation and Bioremediation [M]. Academic Press, San Diego. 1994: 123-170.

Google Scholar

[4] LI Wei, LIU Yanli, CAO Haihua et al. Physiological and biochemical analysis of two moderately halophilic bacteria[J]. Journal of Lanzhou University(Natural Science Edition). Issue 4, 2003: 58-63.

Google Scholar

[5] Aidong Ruan, Hang Min, Xiaohui Peng. Isolation and characterization of Pseudomonas sp. strain HF-1, capable of degrading nicotine[J]. Research in Microbiology, Volume 156, Issues 5-6, June-July 2005: 700-706.

DOI: 10.1016/j.resmic.2005.02.010

Google Scholar

[6] Hana Nováková, Milu e Vo ahlíková, Jarmila Pazlarová et al. PCB metabolism by Pseudomonas sp. P2[J]. International Biodeterioration & Biodegradation, Volume 50, Issue 1, July 2002: 47-54.

DOI: 10.1016/s0964-8305(02)00058-6

Google Scholar

[7] LIANG Siyu, LU Zhaoxin, ZOU Xiaokui et al. The isolation, screening and identification of Bacillus subtilis strains producing high activity fibronolysin[J]. Microbiology. Issue 5, 2001: 25-28.

Google Scholar