In this study, a new method (negative pressure cavitation method, NPC) for metal ions removal from industry waste water has been studied. Under the identical adsorption conditions，we have seen some different effects among static adsorption, shaking adsorption and NPC adsorption. The results are as following: four kind metal ions showed the accordant phenomenon that the sequence of adsorption capacity is NPC adsorption>shaking adsorption>static adsorption. The different metal ions have different improvement of removal ratio. The sequence is Cd>Hg>Pb>Cu. The intercellular pH of absorbed yeast cells increased with the adsorption process from acidic to alkaline. These may suggest that the Candida utilis has certain resistance against heavy metals. The increased pH is also an environment stress response of Candida utilis. So NPC adsorption may have potential values to removal of metal ions.