Biodegradation of Lindane and Lignocellulose by Microbial Community

Abstract:

In order to investigate the capability of degradation lindane and lignocellulose of a microbial community, the gas chromatography(GC) was used to determine the concentration of lindane. After 18 days cultured, lindane reduced 6.25% more than the control. And the result of gas chromatography and mass spectrometry (GCMS) showed that the species and quantum of fermentation productions changed much in different phase. In order to determine the bacterial composition of the community, clone library was used to clarified bacterial composition. Constructed 16S rDNA clone library showed 60% closest relative among them were known the detailed information. Strain of closest relative of Clostridium may be main contribute to lignocellulose degradation, and closest relative of Proteobacterium may be the main contribute to degradation organochlorine pesticide (lindane).

• Periodical: Advanced Materials Research (Volumes 113-116)
• Pages: 55-58
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.113-116.55
• Online since: June 2010
• Authors: Jian Huang, Chang Li Liu, Zong Jun Cui, Hai Long Shen
• Keywords: Biodegradation, Lignocellulose, Lindane

References

[1] Anon, How safe is our bottled water? Down Earth, vol. 11 (2003), pp.27-32.

[2] K.M. Paknikar, V. Nagpal, A.V. Pethkar et al., Degradation of lindane from aqueous solutions using iron sulfide nanoparticles stabilized by biopolymers. Science and Technology of Advanced Materials, vol. 6 (2005), pp.370-374.

DOI: 10.1016/j.stam.2005.02.016

[3] C L Liu, X F Wang, J L Niu, et al. Composition diversity of the multifunctional microbial community NSC-7. Environmental Science, vol. 30(2009), pp.2345-2349.

[4] Hatsumi S, Ikeno S A, Naoaki K, Akiko M, Kuniaki H, Teruhiko B, et al. Isolation and characterization of a new Clostridium sp. that performs effective cellulosic waste digestion in a thermophilic methanogenic bioreactor. AEM, vol. 72(2006).

DOI: 10.1128/aem.72.5.3702-3709.2006

[5] Murashima K, Kosugi A, Doi R H. Synergistic effects of cellulosomal xylanase and cellulases from Clostridium cellulovorans on plant cell wall degradation. J Bacteriol, vol. 185(2003), pp.1518-1524.

DOI: 10.1128/jb.185.5.1518-1524.2003

[6] Cha J, Satoshi M, Helen C, Yukawa H, Inui M. Effect of multiple copies of cohesins on cellulase and hemicellulase activities of Clostridium cellulovorans mini-cellulosomes, J Microbiol Biotechnol, vol. 17 (2007), pp.1782-1788.

[7] Kosugi A Y, Amano K M, Doi R H. Hydrophilic domains of scaffolding protein CbpA promote glycosyl hydrolase activity and localization of cellulosomes to the cell surface of Clostridium cellulovorans. J Bacteriol, vol. 186(2004), pp.6351-6359.

DOI: 10.1128/jb.186.19.6351-6359.2004

[8] M S Takagi, M A Hashida, R H Doi. The hydrophobic repeated domain of the Clostridium cellulovorans cellulose-binding protein (CbpA) has specific interactions with endoglucanases. J Bacteriol, vol. 175(1993), pp.7119-7122.

DOI: 10.1128/jb.175.21.7119-7122.1993