The bench-scale experiments were investigated to optimize chloramines disinfection for Copepod removal by response surface methodology (RSM). Independent variables were chloramines dosage, exposure time and organic matter concentrations. The results showed that the body surface structure provided Copepod with strong protection against disinfection. The independent variables of dosage and exposure time significantly influenced the disinfection rate of Copepod. The disinfection rate was remarkably increased with the increasing chloramines dosage and exposure time. Organic matter concentrations had a less influence on Copepod inactivation. The ANOVA (analysis of variance) data showed that the F-value of 79.99 implied the model significance and the P-value less than 0.0001 indicated its good fit to use in the matrix. The polynomial equation was established by the model coefficient with R2 value of 0.9863. The evaluation proved that the model had a suitable predication for the actual application in waterworks.