Initial Attachment and Biofilm Formation of a Novel Crenarchaeote on Mineral Sulfides

Rui Yong Zhang; Yu Tong Zhang; Thomas R. Neu; Qian Li; Sören Bellenberg; Wolfgang Sand; Mario Vera

doi:10.4028/www.scientific.net/AMR.1130.127

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Industrial Views to Microbe-Metal Interactions in Sub-Arctic Conditions
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Reactive Oxygen Species Influence Biofilm Formation of Acidophilic Mineral-Oxidizing Bacteria on Pyrite
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Characterization of Microbe-Mineral Interfacial Interaction Based on Synchrotron Radiation Techniques
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Initial Attachment and Biofilm Formation of a Novel Crenarchaeote on Mineral Sulfides
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Microbial & Mineralogical Dynamics during Copper Sulfides Bioleaching, the Search for the Missing Link
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Antimicrobial Activity and Hardness of as Cast and as Homogenized Cu-Ti Alloys
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Biofilm Formation of Sulfobacillus thermosulfidooxidans on Pyrite in the Presence of Leptospirillum ferriphilum
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Insight into the Sulfur Metabolism by Thermoacidophilic Archaeon Metallosphaera cuprina with Genomic, Proteomic and Biochemical Tools
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Initial Attachment and Biofilm Formation of a Novel Crenarchaeote on Mineral Sulfides

Abstract:

This study focused on colonization and biofilm formation of a new crenarchaeote Acidianus sp. DSM 29099 on pyrite and chalcopyrite. Confocal laser scanning microscopy (CLSM) in combination with several fluorescent stains was applied to examine spatial distribution of cells and biofilms, as well as extracellular polymeric substances (EPS) production on the substrates. Around 60% and 35% of the inoculum adhered to pyrite and chalcopyrite within 2 h, respectively. Cells of Acidianus sp. DSM 29099 were heterogeneously distributed on both pyrite and chalcopyrite surfaces, while large mineral surfaces remained uncolonized. Biofilm cells on pyrite were often found to be embeded in EPS. EPS residues like mannose and glucose were possibly involved in intial attachment to pyrite. A mature biofilm on pyrite was developed after 2-4 days of incubation.