Reactive Oxygen Species Influence Biofilm Formation of Acidophilic Mineral-Oxidizing Bacteria on Pyrite

Sören Bellenberg; Dieu Huynh; Laura Castro; Maria Boretska; Wolfgang Sand; Mario Vera

doi:10.4028/www.scientific.net/AMR.1130.118

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Reactive Oxygen Species Influence Biofilm Formation of Acidophilic Mineral-Oxidizing Bacteria on Pyrite
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1130Reactive Oxygen Species Influence Biofilm...

Reactive Oxygen Species Influence Biofilm Formation of Acidophilic Mineral-Oxidizing Bacteria on Pyrite

Abstract:

Reactive oxygen species (ROS), such as hydrogen peroxide (H₂O₂), superoxide (O₂^-) and hydroxyl radicals (OH^.) are known to be formed on the surface of metal sulfides in aqueous solution under oxic and anoxic conditions. Consequently bacteria which have not been adapted to their presence are metabolically inhibited [1], presumably due to the presence of these ROS. Pyrite-grown cells of Acidithiobacillus ferrooxidans^T, in contrast to iron (II)-grown cells, were able to oxidize iron (II)-ions or pyrite after 24 h starvation and contact with 1 mM externally added H₂O₂. In this study, similar results were obtained with Acidiferrobacter sp. SPIII/3. However, Acidithiobacillus ferrivorans SS3 showed the highest tolerance towards contact with H₂O₂, while Leptospirillum ferrooxidans DSM 2391 was most sensitive. Similar results were obtained after exposure to defined doses of gamma radiation, which cleaves water molecules and generates ROS. In this study members of the three aforementioned genera of mineral-oxidizing bacteria were compared regarding their ability to survive, colonize pyrite and to oxidize iron (II)-ions after exposure to different concentrations of H₂O₂. Pyrite colonization was studied after exposure to endogenous ROS formed on pyrite or after external addition of H₂O₂ using confocal laser scanning microscopy (CLSM).

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Periodical:

Advanced Materials Research (Volume 1130)

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118-122

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https://doi.org/10.4028/www.scientific.net/AMR.1130.118

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Online since:

November 2015

Authors:

Sören Bellenberg*, Dieu Huynh, Laura Castro, Maria Boretska, Wolfgang Sand, Mario Vera

Keywords:

Biofilm Formation, Bioleaching, Pyrite, Reactive Oxygen Species

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References

[1] S. Bellenberg, R. Barthen, M. Boretska, R. Zhang, W. Sand, M. Vera, Manipulation of pyrite colonization and leaching by iron-oxidizing Acidithiobacillus species, Appl. Microbiol. Biotechnol. 99 (2015) 1435-1449.

DOI: 10.1007/s00253-014-6180-y

Google Scholar

[2] G. C. Jones, K. C. Corin, R. P. van Hille, S. T. L. Harrison, The generation of toxic reactive oxygen species (ROS) from mechanically activated sulphide concentrates and its effect on thermophilic bioleaching, Miner. Eng. 24 (2011) 1198-1208.

DOI: 10.1016/j.mineng.2011.05.016

Google Scholar

[3] M. Dopson, F. J. Ossandon, L. Lövgren, D. S. Holmes, Metal resistance or tolerance? Acidophiles confront high metal loads via both abiotic and biotic mechanisms, Front. Microbiol. (2014) doi: 10. 3389/fmicb. 2014. 00157.

DOI: 10.3389/fmicb.2014.00157

Google Scholar

[4] M. Mackintosh, Nitrogen fixation by Thiobacillus ferrooxidans, J. Gen. Microbiol. 105 (1978) 215-218.

DOI: 10.1099/00221287-105-2-215

Google Scholar

[5] A. Schippers, P. Jozsa, W. Sand, Sulfur chemistry in bacterial leaching of pyrite, Appl. Environ. Microbiol. 62 (1996) 3424-3431.

DOI: 10.1128/aem.62.9.3424-3431.1996

Google Scholar

[6] DEV (1989) DIN 38 406: Kationen (Gruppe E), Teil 1 Bestimmung von Eisen (E1), Normenausschuss Wasserwesen im Deutschen Institut für Normierung eV: 22 Lieferung (1989).

Google Scholar

[7] J. L. Oblinger and J. A. Koburger, Understanding and Teaching the Most Probable Number Technqiue, J. Milk Food Technol. 38 (1975) 540-545.

DOI: 10.4315/0022-2747-38.9.540

Google Scholar

[8] S. Bellenberg, C. F. Leon-Morales, W. Sand and M. Vera, Visualization of capsular polysaccharide induction in Acidithiobacillus ferrooxidans, Hydrometallurgy 129-130 (2012) 82-89.

DOI: 10.1016/j.hydromet.2012.09.002

Google Scholar