Extraction and Characterization of Galactomannan from Seeds of Leucaena leucocephala

Abstract:

Galactomannan is a biopolymer polysaccharides mostly obtained from Leguminosae plant have shown an interesting capability as drug carrier. In this study, galactomannan from the seeds of Leucaena leucocephala known locally as ‘petai belalang’ was obtained by water extraction. The galactomannan was characterized structurally by nuclear magnetic resonance (NMR) and fourier transform infrared spectroscopy (FTIR). Highly molecular weight of galactomannan, > 2.56 x 10⁶ Da, was successfully extracted from the plant. Total carbohydrate content of this polysaccharides is 74.84% and composition of galactose and mannose have been confirm by high performance liquid chromatography (HPLC) which is 36.67% and 38.17% respectively. M/G ratio was determined as 1:1. Hence, galactomannan has been successfully extracted from the seeds of Leucaena leucocephala.

• Periodical: Advanced Materials Research (Volume 1134)
• Pages: 213-219
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1134.213
• Online since: December 2015
• Authors: Shazlinda Shirajuddin*, Dzaraini Kamarun, Nahlah Elkudssiah Ismail, Mohd Shahezwan Abdul Wahab, Abdul Rashid Li, Nurnadiah Rahim
• Keywords: Galactomannan, Leguminosae, Leucaena leucocephala

References

[1] P. B. S. Albuquerque, W. Barros, G. R. C. Santos, M. T. S. Correia, P. A. S. Mourao, J. A. Teixeira, and M. G. Carneiro-da-Cunha, Characterization and rheological study of the galactomannan extracted from seeds of Cassia grandis, Carbohydr. Polym. 104, (2014).

DOI: 10.1016/j.carbpol.2014.01.010

[2] J. K. Suh and H. W. Matthew, Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering, Biomaterials. 21, (2000) 2589–2598.

DOI: 10.1016/s0142-9612(00)00126-5

[3] M. S. Buckeridge, S. M. C. Dietrich, and D. U. De Lima, Galactomannans as the reserve carbohydrate in legume seeds, Dev. Crop Sci. 26, (2000) 283–316.

DOI: 10.1016/s0378-519x(00)80015-x

[4] P. L. R. Cunha, I. G. P. Vieira, A. M. C. Arriaga, R. C. M. de Paula, and J. P. A. Feitosa, Isolation and characterization of galactomannan from Dimorphandra gardneriana Tul. seeds as a potential guar gum substitute, Food Hydrocoll. 23 (2009).

DOI: 10.1016/j.foodhyd.2008.05.005

[5] C. W. Vendruscolo, C. Ferrero, E. A. G. Pineda, J. L. M. Silveira, R. A. Freitas, M. R. Jimenez-Castellanos, and T. M. B. Bresolin, Physicochemical and mechanical characterization of galactomannan from Mimosa scabrella: Effect of drying method, Carbohydr. Polym. 76 (2009).

DOI: 10.1016/j.carbpol.2008.09.028

[6] M. Shobha, A. Vishukumar, R. Tharanathan, R. Koka, and A. Gaonkar, Modification of guar galactomannan with the aid of pectinase, Carbohydr. Polym. 62 (2005) 267–273.

DOI: 10.1016/j.carbpol.2005.07.031

[7] R. R. de Almeida, H. S. Magalhaes, J. R. R. de Souza, M. T. S. Trevisan, I. G. P. Vieira, J. P. A. Feitosa, T. G. Araujo, and N. M. P. S. Ricardo, Exploring the potential of Dimorphandra gardneriana galactomannans as drug delivery systems, Ind. Crops Prod. 69 (2015).

DOI: 10.1016/j.indcrop.2015.02.041

[8] Y. S. R. Krishnaiah, S. Satyanarayana, Y. V. R. Prasad, and S. N. Rao, Evaluation of guar gum as a compression coat for drug targeting to colon, Int. J. Pharm. 171 (1998) 137–146.

DOI: 10.1016/s0378-5173(98)00172-0

[9] B. S. Kaith, R. Sharma, and S. Kalia, Guar gum based biodegradable, antibacterial and electrically conductive hydrogels, Int. J. Biol. Macromol. 75 (2015) 1–10.

DOI: 10.1016/j.ijbiomac.2015.01.046

[10] M. D. O. Salvalaggio, R. A. De Freitas, E. M. Franquetto, H. S. Koop, and J. L. M. Silveira, Influence of the extraction time on macromolecular parameters of galactomannans, Carbohydr. Polym. (2014).

DOI: 10.1016/j.carbpol.2014.05.036

[11] H. A. Pawar and K. G. Lalitha, Isolation, purification and characterization of galactomannans as an excipient from Senna tora seeds., Int. J. Biol. Macromol. 65 (2014) 167–75.

DOI: 10.1016/j.ijbiomac.2014.01.026

[12] L. M. Nwokocha and P. A. Williams, Isolation and characterization of a novel polysaccharide from seeds of Peltophorum pterocarpum, Food Hydrocoll. (2014) 319–324.

DOI: 10.1016/j.foodhyd.2014.04.016

[13] L. M. Nwokocha and P. A. Williams, Rheological characterization of the galactomannan from Leucaena leucocephala seed., Carbohydr. Polym. 90 (2012) 833–8.

DOI: 10.1016/j.carbpol.2012.06.008

[14] M. A. Cerqueira, A. C. Pinheiro, B. W. S. Souza, A. M. P. Lima, C. Ribeiro, C. Miranda, J. A. Teixeira, R. A. Moreira, M. A. Coimbra, M. P. Goncalves, and A. A. Vicente, Extraction, purification and characterization of galactomannans from non-traditional sources, Carbohydr. Polym. 75 (2009).

DOI: 10.1016/j.carbpol.2008.07.036

[15] K. S. Parvathy, N. S. Susheelamma, and R. N. Tharanathan, Hydration characteristics of guar gum samples and their fractions, Food Hydrocoll. 21 (2007) 630–637.

DOI: 10.1016/j.foodhyd.2006.07.006

[16] A. M. Unrau, The constitution of a galactomannan from the seed of Leucaena glauca, Org. Chem. 26 (1961) 3097–3101.

DOI: 10.1021/jo01067a013

[17] M. M. Hussein, W. A. Helmy, and H. M. Salem, Biological activities of some galacomannans and their sulfated derivaties, Phytochemistry. 48 (1998) 479-484.

DOI: 10.1016/s0031-9422(98)00024-7

[18] B. Torrestiana-Sanchez, L. Balderas-Luna, E. Brito-De la Fuente, and R. W. Lencki, The use of membrane-assisted precipitation for the concentration of xanthan gum, J. Memb. Sci. 294 (2007) 84–92.

DOI: 10.1016/j.memsci.2007.02.014

[19] M. A. Cerqueira, B. W. S. Souza, J. Simoes, J. A. Teixeira, M. R. M. Domingues, M. A. Coimbra, and A. A. Vicente, Structural and thermal characterization of galactomannans from non-conventional sources, Carbohydr. Polym. 83 (2011) 179–185.

DOI: 10.1016/j.carbpol.2010.07.036

[20] H. Kono and S. Fujita, Biodegradable superabsorbent hydrogels derived from cellulose by esterification crosslinking with 1, 2, 3, 4-butanetetracarboxylic dianhydride, " Carbohydr. Polym. 87 (2012) 2582–2588.

DOI: 10.1016/j.carbpol.2011.11.045

[21] H. Kono, F. Otaka, and M. Ozaki, Preparation and characterization of guar gum hydrogels as carrier materials for controlled protein drug delivery, Carbohydr. Polym. 111 (2014) 830–840.

DOI: 10.1016/j.carbpol.2014.05.050

[22] O. Ishrud, M. Zahid, H. Zhou, and Y. Pan, "A water-soluble galactomannan from the seeds of Pheonix dactylifera L., Carbohydr. Res. 335 (2001) 297–301.

DOI: 10.1016/s0008-6215(01)00245-2

[23] S. Omarsdottir, B. O. Petersen, H. Barsett, B. S. Paulsen, J. Ø. Duus, and E. S. Olafsdottir, Structural characterisation of a highly branched galactomannan from the lichen Peltigera canina by methylation analysis and NMR-spectroscopy, Carbohydr. Polym. 63 (2006).

DOI: 10.1016/j.carbpol.2005.07.023

[24] M. A. Pollard, B. Eder, P. Fischer, and E. J. Windhab, Characterization of galactomannans isolated from legume endosperms of Caesalpinioideae and Faboideae subfamilies by multidetection aqueous SEC, Carbohydr. Polym. 79 (2010) 70–84.

DOI: 10.1016/j.carbpol.2009.07.028

[25] F. Ughini, I. F. Andreazza, J. L. M. S. Ganter, and T. M. B. Bresolin, Evaluation of xanthan and highly substituted galactomannan from M . scabrella as a sustained release matrix, 271 (2004) 197–205.

DOI: 10.1016/j.ijpharm.2003.11.011

[26] J. L. M. Silveira and T. M. B. Bresolin, Pharmaceutical use of galactomannans. 34 (2011) 292–299.