The multi-body system analysis has become one of the main simulation techniques to calculate the kinematics characteristics of a car suspension under wheel travel or to build a virtual prototype model of a vehicle in order to predict the vehicle dynamics performance. The modeling of twist beam rear suspensions is always difficult for the unique structural behavior of this component. A non-linear method based on multi-body dynamics software (ADAMS/Car) was used to represent the twist beam within the suspension system. The kinematics characteristic analysis of the rear suspension was realized by means of ADAMS/Car. The main suspension parameters (toe angle, camber angle and wheel track variation) were calculated by changing wheel travel. The result shows that the method of suspension kinematics analysis by using ADAMS/Car can be used in the design of suspensions conveniently.