A research on measurement method of space position in three-dimensional field was demonstrated in this paper. According to the basic methods and principles of three-dimensional spatial position measurement, optical path of splitting design was used to divide an object point, through the red and green filters, into two beams of red and green, and then, through flat mirror, rectangular prism, cube prism and other optical design, it imaged on the same CCD camera film. Standard mesh was used to determine the relationship between two different spatial coordinates and the spatial pixel coordinates in the measurement space, and then the calibration relationship between object point coordinates and pixel coordinates were determined. This calibration relationship clear the error caused by the method of measurement and the measurement system. Through the determination algorithm of spatial location in three-dimensional field, the two corresponding lines of space was obtained, and its point of intersection is the spatial coordinate.