The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Phosphate/Polyacrylate/Alginate Hybrid Polymer Microspheres

Kong Yin Zhao; Jun Fu Wei; Jin Yang Zhou; Yi Ping Zhao; Guo Xiang Cheng

doi:10.4028/www.scientific.net/AMR.152-153.1636

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 152-153The Rebinding Properties of Bovine Serum Albumin...

The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Phosphate/Polyacrylate/Alginate Hybrid Polymer Microspheres

Abstract:

Calcium phosphate/polyacrylate/alginate hybrid polymer microspheres with bovine serum albumin (BSA) embedded and coated on the surface were prepared with (NH4)2HPO4, sodium polyacrylate (SPA) and sodium alginate (SA) via Ca2+ crosslinking in inverse suspension. Rebinding behaviors of the microspheres were evaluated. The factors influencing the imprinting efficiency (IE) of imprinted microspheres were also studied, including the concentration of CaCl2, template content and pH values in rebinding solutions. Selectivity tests showed that the imprinted microspheres exhibited good recognition property for the template protein.

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Periodical:

Advanced Materials Research (Volumes 152-153)

Pages:

1636-1640

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.152-153.1636

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Online since:

October 2010

Authors:

Kong Yin Zhao, Jun Fu Wei, Jin Yang Zhou, Yi Ping Zhao, Guo Xiang Cheng

Keywords:

Alginate, Bovine Serum Albumin (BSA), Hybrid Hydrogel, Molecular Imprinting, Rebinding

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