Characteristics of Biohydrogen Production by Ethanoligenens Sp. R3 Isolated from Continuous Stirred Tank Reactor

Abstract:

Ethanoligenens sp. R3 isolated from anaerobic sludge in a continuous stirred tank reactor was investigated for its characteristics of fermentative hydrogen production in this study. Effects of initial pH value, fermentation temperature and proportion of carbon/nitrogen source on hydrogen production were investigated in batch experiments. The typical substrate for hydrogen production of glucose and peptone were used as the carbon source and nitrogen source, respectively. The results show that the maximum hydrogen production yield of 834 mlH2/L culture was obtained with initial pH value of 5.5 and fermentation temperature at 35°C. In addition, the maximum average hydrogen production rate of 10.87mmolH2/g cell dry weighth was achieved at the carbon/nitrogen source ratio of 3.3. The degradation efficiency of the glucose used as the carbon source ranged from 91.5% to 95.43% during the conversion of glucose to hydrogen by bacteria Ethanoligenens sp. R3.

• Periodical: Advanced Materials Research (Volumes 152-153)
• Pages: 702-707
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.152-153.702
• Online since: October 2010
• Authors: An Ying Jiao, Yong Feng Li, Bing Liu, Kun Liu, Jing Li Xu
• Keywords: Fermentative Hydrogen Production, Hydrogen Yield, Initial pH Value

References

[1] Chmiel, M. T. and V. Yargeau. Biohydrogen production by Clostridium beijerinckii. Waste Management and the Environment Iv. Vol. 109 (2008), pp.123-132.

DOI: 10.2495/wm080141

[2] Kotay, S. M. and D. Das. Biohydrogen as a renewable energy resource-Prospects and potentials. Int. J. of Hydrogen Energy. Vol. 33(2008), pp.258-263.

DOI: 10.1016/j.ijhydene.2007.07.031

[3] Blackburn, J., Y. Liang, et al. Biohydrogen from complex carbohydrate wastes as feedstocks-Cellulose degraders from a unique series enrichment. Int. J. of Hydrogen Energy. Vol. 34(2009), pp.7428-7434.

DOI: 10.1016/j.ijhydene.2009.04.014

[4] Azbar, N., F. T. C. Dokgoz, et al. Continuous fermentative hydrogen production from cheese whey wastewater under thermophilic anaerobic conditions. Int. J. of Hydrogen Energy. Vol. 34 (2009), pp.7441-7447.

DOI: 10.1016/j.ijhydene.2009.04.032

[5] Manish, S. and R. Banerjee. Comparison of biohydrogen production processes. Int. J. of Hydrogen Energy. Vol. 33 (2008), pp.279-286.

DOI: 10.1016/j.ijhydene.2007.07.026

[6] Das, D. Advances in biohydrogen production processes: An approach towards commercialization. Int. J. of Hydrogen Energy. Vol. 34(2009), pp.7349-7357.

DOI: 10.1016/j.ijhydene.2008.12.013

[7] Krupp, M. and R. Widmann. Biohydrogen production by dark fermentation: Experiences of continuous operation in large lab scale. Int. J. of Hydrogen Energy. Vol. 34(2009), pp.4509-4516.

DOI: 10.1016/j.ijhydene.2008.10.043

[8] Chmiel, M. T. and V. Yargeau. Biohydrogen production by Clostridium beijerinckii. Waste Management and the Environment Iv. Vol. 109(2008), pp.123-132.

DOI: 10.2495/wm080141

[9] Ganeshkumar, A. and S. V. Vikash. Biohydrogen Production from Molasses Using Wild Type E. Coli. Research Journal of Biotechnology. (2008), pp.194-195.

[10] Jo, J. H., D. S. Lee, et al. Statistical optimization of key process variables for enhanced hydrogen production by newly isolated Clostridium tyrobutyricum JM1. Int. J. of Hydrogen Energy. Vol. 33(2008), pp.5176-5183.

DOI: 10.1016/j.ijhydene.2008.05.012

[11] Jianlong Wang, Wei Wan Factors influencing fermentative hydrogen production: A review. Int. J. of Hydrogen Energy. Vol. 34(2009), pp.799-811.

DOI: 10.1016/j.ijhydene.2008.11.015

[12] Koukios, E., D. Koullas, et al. Critical parameters for optimal biomass refineries: the case of biohydrogen. Clean Technologies and Environmental Policy. Vol. 12(2010), pp.147-151.

DOI: 10.1007/s10098-009-0239-y

[13] Xiaomin Wu, Honghui Yang, Liejin Guo. Effect of operation parameters on anaerobic fermentation using cow dung as a source of microorganisms. Int. J. of Hydrogen Energy. Vol. 35(2010), pp.46-51.

DOI: 10.1016/j.ijhydene.2009.10.039

[14] Ozgur, E., B. Uyar, et al. Biohydrogen production by Rhodobacter capsulatus on acetate at fluctuating temperatures. Resources Conservation and Recycling. Vol. 54(2010), pp.310-314.

DOI: 10.1016/j.resconrec.2009.06.002

[15] Wang J.L., Wan W. Effect of temperature on fermentative hydrogen production by mixed cultures. Int J Hydrogen Energy. Vol. 33( 2008), pp.5392-5397.

DOI: 10.1016/j.ijhydene.2008.07.010

[16] Salerno M.B., Park W., Zuo Y., Logan B.E. Inhibition of biohydrogen production by ammonia. Water Res. Vol. 40( 2006), pp.1167-1172.

DOI: 10.1016/j.watres.2006.01.024

[17] R.S. Prakasham, P. Brahmaiah, T. Sathish , K.R.S. Sambasiva Rao. Fermentative biohydrogen production by mixed anaerobic consortia: Impact of glucose to xylose ratio. Int. J. of Hydrogen Energy. Vol. 34(2009), pp.9354-9361.

DOI: 10.1016/j.ijhydene.2009.09.104

[18] Bisaillon A., Turcot J, Hallenbeck P.C. The effect of nutrient limitation on hydrogen production by batch cultures of Escherichia coli. Int. J. of Hydrogen Energy. Vol. 31(2006), pp.1504-1508.

DOI: 10.1016/j.ijhydene.2006.06.016