The shape and size of the linked polymer microspheres and the matching between the microsphere size and nuclear-pore membranes were studied in this paper. Scanning electron microscopy(SEM), microscope, dynamic lighting scattering(DLS), Laser diffracting measurements, nuclear-pore film filtration and sand packed tube displacement experiment was adopted for investigation. The results showed that the original shape of the microspheres is typically spherical with a size range of 400-5000nm. When the microspheres were dispersed in water, swelling occurred, resulting in larger size but spherical conformation remained. And the system was poly-dispersed. There are certain matching relationships between the microsphere size and the membranes pore size, and the best plugging property could only occur when they are well-matched. Matching relationships also exist in the sand packed tube displacement experiment, when sand packed tubes with low permeability were used, plugging could occur effectively, and well in-depth plugging could happen as well.