Fabrication, Characterization and Adsorptive of Human Fibrinogen of HA/PHBV Nano Composite Films

Abstract:

In this investigation, nano-scale hydroxyapatite (HA) particles were incorporated in poly(hydroxybutyrate-co-valerate) (PHBV) polymer to fabricate composite films using solvent casting method. The morphologies, mechanical properties and adsorptive of human fibrinogen of the composite films were investigated. The HA nano-particles were dispersed in the films and well bonded to the polymer. The strength of films could be enhanced and its adsorptive of human fibrinogen could be also improved greatly by introducing HA nano-particles into the films. The results suggested these newly developed nano-HA/PHBV composite films might serve as an effective biomedicine material.

• Periodical: Advanced Materials Research (Volumes 160-162)
• Pages: 1325-1330
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.160-162.1325
• Online since: November 2010
• Authors: Jun Yuan, Qi Xin Zheng, Jian Cheng
• Keywords: Human Fibrinogen, Hydroxyapatite (HAP), Poly(hydroxybutyrate-co-valerate) (PHBV), Solvent Casting Method

References

[1] Sodian, R., S. Hoerstrup, et al. Evaluation of Biodegradable, Three-Dimensional Matrices for Tissue Engineering of Heart Valves., Asaio Journal , 2000, 46(1): 107-110.

DOI: 10.1097/00002480-200001000-00025

[2] Iordanskii, A. L., P. P. Kamaev, et al. Immobilization influence on the water sorption and diffusion in poly(3-hydroxybutyrate)., Journal of Applied Polymer Science , 1999, 73(6): 981-985.

DOI: 10.1002/(sici)1097-4628(19990808)73:6<981::aid-app16>3.0.co;2-y

[3] Sendil, D., I. Gürsel, et al. Antibiotic release from biodegradable PHBV microparticles., Journal of Controlled Release, 1999, 59(2): 207-217.

DOI: 10.1016/s0168-3659(98)00195-3

[4] Torun Köse, G., S. Ber, et al. Poly(3-hydroxybutyric acid-co- 3-hydroxyvaleric acid) based tissue engineering matrices., Journal of Materials Science: Materials in Medicine , 2003, 14(2): 121-126.

[5] Tesema, Y., D. Raghavan, et al. Bone cell viability on collagen immobilized poly (3-hydroxybutrate-co-3-hydroxyvalerate) membrane: Effect of surface chemistry., Journal of Applied Polymer Science, 2004, 93(5): 2445-2453.

DOI: 10.1002/app.20787

[6] LeGeros, R. Properties of osteoconductive biomaterials: calcium phosphates., Clin Orthop Relat Res, 2002, 395: 81-98.

DOI: 10.1097/00003086-200202000-00009

[7] Kurashina, K., H. Kurita, et al. Ectopic osteogenesis with biphasic ceramics of hydroxyapatite and tricalcium phosphate in rabbits., Biomaterials, 2002, 23(2): 407-412.

DOI: 10.1016/s0142-9612(01)00119-3

[8] Fathi, M. H. and A. Hanifi. Evaluation and characterization of nanostructure hydroxyapatite powder prepared by simple sol-gel method., Materials Letters, 2007, 61(18): 3978-3983.

DOI: 10.1016/j.matlet.2007.01.028

[9] Kim, W., Q. Zhang, et al. Mechanochemical synthesis of hydroxyapatite from Ca(OH)2-P2O5 and CaO-Ca(OH)2-P2O5 mixtures., Journal of Materials Science, 2000, 35(21): 5401-5405.

DOI: 10.3390/nano10112232

[10] Tas, A. C. Combustion synthesis of calcium phosphate bioceramic powders., Journal of the European Ceramic Society, 2000, 20(14-15): 2389-2394.

DOI: 10.1016/s0955-2219(00)00129-1

[11] Tas, A. C. Synthesis of biomimetic Ca-hydroxyapatite powders at 37°C in synthetic body fluids., Biomaterials, 2000, 21(14): 1429-1438.

DOI: 10.1016/s0142-9612(00)00019-3

[12] López-Macipe, A., R. Rodríguez-Clemente, et al. Wet Chemical Synthesis of Hydroxyapatite Particles from Nonstoichiometric Solutions., Journal of Materials Synthesis and Processing, 1998, 6(1): 21-26.

[13] Huang, L. -Y., K. -W. Xu, et al. A study of the process and kinetics of electrochemical deposition and the hydrothermal synthesis of hydroxyapatite coatings., Journal of Materials Science: Materials in Medicine, 2000, 11(11): 667-673.

[14] Wenjian, W. and J. L. Baptista. Alkoxide route for preparing hydroxyapatite and its coatings., Biomaterials, 1998, 19(1-3): 125-131.

DOI: 10.1016/s0142-9612(97)00177-4

[15] Yoshimura, M., H. Suda, et al. Hydrothermal synthesis of biocompatible whiskers., Journal of Materials Science, 1994, 29(13): 3399-3402.

DOI: 10.1007/bf00352039

[16] Lim, G., J. Wang, et al. Nanosized hydroxyapatite powders from microemulsions and emulsions stabilized by a biodegradable surfactant., Journal of Materials Chemistry, 1999, 9(7): 1635-1639.

DOI: 10.1039/a809644i

[17] Zhou, Y. X., B. Liu, et al. Preparation of Nano-hydroxyapatite in Impinging Stream Reactor., Chemistry & Bioengineering, 2007, 24(06): 15-17.

[18] Witte, F., F. Feyerabend, et al. Biodegradable magnesium -hydroxyapatite metal matrix composites., Biomaterials, 2007, 28(13): 2163-2174.

DOI: 10.1016/j.biomaterials.2006.12.027

[19] Ge, Z., Baguenard, A.S., Lim, L.Y., Wee, A., Khor, E. Hydroxyapatite -chitin materials as potential tissue engineered bone substitute, Biomaterials, 2004, 25: 1049-1058.

DOI: 10.1016/s0142-9612(03)00612-4

[20] Kaito, T., A. Myoui, et al. Potentiation of the activity of bone morphogenetic protein-2 in bone regeneration by a PLA-PEG /hydroxyapatite composite., Biomaterials, 2005, 26(1): 73-79.

DOI: 10.1016/j.biomaterials.2004.02.010

[21] Tezcaner, A., K. Bugra, et al. Retinal pigment epithelium cell culture on surface modified poly(hydroxybutyrate-co-hydroxyvalerate) thin films., Biomaterials, 2003, 24(25): 4573-4583.

DOI: 10.1016/s0142-9612(03)00302-8

[22] Song, L., J. Meng, et al. Human fibrinogen adsorption onto single-walled carbon nanotube films., Colloids and Surfaces B: Biointerfaces, 2006, 49(1): 66-70.

DOI: 10.1016/j.colsurfb.2005.12.003

[23] Wang, Y., X. Wang, et al. Fabrication, characterization and long-term in vitro release of hydrophilic drug using PHBV/HA composite microspheres., Materials Letters, 2007, 61(4-5): 1071-1076.

DOI: 10.1016/j.matlet.2006.06.062