In this investigation, nano-scale hydroxyapatite (HA) particles were incorporated in poly(hydroxybutyrate-co-valerate) (PHBV) polymer to fabricate composite films using solvent casting method. The morphologies, mechanical properties and adsorptive of human fibrinogen of the composite films were investigated. The HA nano-particles were dispersed in the films and well bonded to the polymer. The strength of films could be enhanced and its adsorptive of human fibrinogen could be also improved greatly by introducing HA nano-particles into the films. The results suggested these newly developed nano-HA/PHBV composite films might serve as an effective biomedicine material.