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Full Length cDNA Cloning and Expression Characteristics of Ace Gene from Wild Silkworm, Bombyx mandarina
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Expression Profiles of Glutathione S-Transferase Genes in Bombyx mori Exposed to NaF
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Expression Profiles of P450 4 Family Genes in Larval Fatbody and Midgut of Bombyx Mori Exposed to Rutin
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Standardization of Reference Genes in Silkworm, Bombyx Mori
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Expression Profiles of Glutathione S-Transferase Genes in Bombyx mori Exposed to NaF

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Abstract:

In order to explore the roles of Bombyx mori glutathione S-transferase gene (BmGST) in detoxification and resistance to insecticides, we used real-time PCR method to detect the transcription levels of five BmGST genes in different tissues of the 5th instar larvae feeding on sodium fluoride treated mulberry leaves. The detection results were normalized by using 3 internal reference genes. The results indicated that the transcription levels of BmGSTs were different in various tissues. Transcription of BmGST genes could be induced by NaF, The normalized data with the above 3 internal reference genes indicated that, it is very important to choose adequate internal reference genes so as to ensure the reliability of the detection results.

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Periodical:

Advanced Materials Research (Volumes 175-176)

Pages:

56-60

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.175-176.56

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Online since:

January 2011

Authors:

Guo Dong Zhao, Yi Ling Zhang, Rui Xian Wang, Si Si Zhao, Ran Peng, Bao Jin Su, Bing Li, Yu Hua Chen, Wei De Shen, Zheng Guo Wei

Keywords:

BmGST Genes, Bombyx mori, Induced Expression, Quantified Transcription Level, Tissue-Specific Expression

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© 2011 Trans Tech Publications Ltd. All Rights Reserved

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