Optimization of Fermentation Conditions for Cold-Active Cellulase Production by Response Surface Methodology

Shuo Dong; Nai Yu Chi; Qing Fang Zhang

doi:10.4028/www.scientific.net/AMR.183-185.1025

Paper Titles

Coagulant Optimization and Effect Research of Low-Temperature and High-Color Source Water Treatment
p.1004

Influence of Bio-Filter Bed on Treatment of Domestic Sewage in Purifying Tank
p.1009

Characterization of amoA and hao Genes Responsible for Ammonia Oxidation Reaction in CANON System
p.1014

Elucidation of Accumulation Mechanism of Environmental Pollutants into Living Body and Defense Mechanism from Health Damage with Chemicals
p.1020

Optimization of Fermentation Conditions for Cold-Active Cellulase Production by Response Surface Methodology
p.1025

Study on the Diffusion Equations of Water Pollutants in Various Water Areas with Different Waterflow States
p.1030

Decolorization of Indigo Carmine by Ganoderma weberianum
p.1035

Emissions of Isoprenoid from Major Planting Tree Species in Shenyang
p.1041

Adsorption Removal of Phosphorus from Aqueous Solution by the Activated Carbon Prepared from Sugarcane Bagasse
p.1046

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 183-185Optimization of Fermentation Conditions for...

Optimization of Fermentation Conditions for Cold-Active Cellulase Production by Response Surface Methodology

Abstract:

The design of an optimum and cost-efficient fermentation conditions for production of cold-active cellulase by Penicillium cordubense D28 was attempted by using response surface methodology (RSM). Based on the Plackett–Burman design, temperature, seed age and inoculum size were selected as the most critical condition. Subsequently, they were investigated by the Box-Behnken design. Results showed that the maximum cold-active cellulase activity of 110.4U/mL was predicted when the coded values of temperature, seed age and inoculum size were -0.495, 0.455, and 0.253, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective temperature, seed age and inoculum size of 17°C, 29h and 12.5%. The maximum cold-active cellulase activity of 219.9 U/mL was obtained based on the optimized fermentation conditions with further verified the practicability of this optimum strategy.

You might also be interested in these eBooks

Environmental Biotechnology and Materials Engineering

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 183-185)

Pages:

1025-1029

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.183-185.1025

Citation:

Cite this paper

Online since:

January 2011

Authors:

Shuo Dong, Nai Yu Chi, Qing Fang Zhang

Keywords:

Cold-Active Cellulase, Fermentation Condition, Penicillium cordubense, Response Surface Method (RSM)

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Maryam Latifian, Zohreh Hamidi-Esfahani, Mohsen Barzegar: Bioresource Technology. Vol. 98(2007), p.3634.

Google Scholar

[2] Krogh, K.B.R.M., Harris, P.V., Olsen, C.L., Johansen, K.S., Pedersen, J.H., Borjesson, J., Olsson, L: Microbiol. Biotechnol. Vol. 86(2010), p.143.

Google Scholar

[3] Chen Liang, Chi Naiyu, Zhang Qingfang: Microbiology China. Vol. 36(2009), p.1547.

Google Scholar

[4] Majumder, A., Goyal, A: Microbiol. Biotechnol. Vol. 74(2007), p.78.

Google Scholar

[5] Miller, G. L: Anal. Chem. Vol. 31(1959), p.426.

Google Scholar

[6] Li, Y.Q., Jiang, H.X., Xu, Y.Q., Zhang, X. H: Microbiol. Biotechnol. Vol. 77(2008), p.1207.

Google Scholar

[7] Liu, J.Z., Weng, L.P., Zhang, Q. L: Microbiol. Biotechnol. Vol. 19(2003), p.317.

Google Scholar