Plant Regeneration in Sorbus. pohuashanenesis Hedl by Somatic Embryogenesis

Abstract:

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Somatic embryogenesis was obtained by using immature zygotic embryos of S. pohuashanesis as explants and emblings were obtained. For induction of somatic embryos, immature zygotic embryos which 30 days old after pollination were cultured on solid MS medium with 1.0 mg•L-1 NAA, 0.1 mg•L-1 6-BA, 500 mg•L-1casein hydrolysate (CH) and 40 g•L-1 sucrose . Inducted somatic embryos were cultured in solid MS medium containing 500 mg•L-1CH and 40 g•L-1 sucrose. After 30 days of culture, many normal cotyledonary embryos were produced. Plantlets were regenerated when somatic embryos were transferred to MS medium with 30 g•L-1 sucrose. The somatic embryos germinated at a germination frequency of approximately 80%, but rate of the plantlets that successfully acclimated and continued growing was 40% in the greenhouse.

Info:

Periodical:

Advanced Materials Research (Volumes 183-185)

Edited by:

Yanguo Shi and Jinlong Zuo

Pages:

1462-1466

DOI:

10.4028/www.scientific.net/AMR.183-185.1462

Citation:

L. Yang et al., "Plant Regeneration in Sorbus. pohuashanenesis Hedl by Somatic Embryogenesis", Advanced Materials Research, Vols. 183-185, pp. 1462-1466, 2011

Online since:

January 2011

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Price:

$35.00

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