Plant Regeneration in Sorbus. pohuashanenesis Hedl by Somatic Embryogenesis

Abstract:

Somatic embryogenesis was obtained by using immature zygotic embryos of S. pohuashanesis as explants and emblings were obtained. For induction of somatic embryos, immature zygotic embryos which 30 days old after pollination were cultured on solid MS medium with 1.0 mg•L^-1 NAA, 0.1 mg•L^-1 6-BA, 500 mg•L^-1casein hydrolysate (CH) and 40 g•L^-1 sucrose . Inducted somatic embryos were cultured in solid MS medium containing 500 mg•L^-1CH and 40 g•L^-1 sucrose. After 30 days of culture, many normal cotyledonary embryos were produced. Plantlets were regenerated when somatic embryos were transferred to MS medium with 30 g•L^-1 sucrose. The somatic embryos germinated at a germination frequency of approximately 80%, but rate of the plantlets that successfully acclimated and continued growing was 40% in the greenhouse.

• Periodical: Advanced Materials Research (Volumes 183-185)
• Pages: 1462-1466
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.183-185.1462
• Online since: January 2011
• Authors: Ling Yang, Yu Hua Li, Hai Long Shen
• Keywords: Plantlet Regeneration, Somatic Embryogenesis, Sorbus. pohuashanenesis, Tissue Culture

References

[1] S.Q. Nie, X.Y. Yuan and F.J. Yang, in: Flora of plant resources in Heilongjiang province, edtied by Northeast Forestry University Publising, Harbin (2003), in Press, pp.387-388.

[2] Z.J. Mo. Sorbus Pohuanensis (Hance) Hedl . var. amrensis (Koehue). Special economical zoology and botany, Forum Vol. 4 (2001), p.25.

[3] U. Oster, I. Blos and W. Rudiger. Natural inhibitors of germination and growth. IV. Compounds from fruit and seeds of mountain ash (Sorbus aucuparia). Zeitschrift Fur Naturforschung, Forum Vol. 42(1987), pp.1179-1184.

DOI: 10.1515/znc-1987-11-1207

[4] T.R. Paulsen and G. Hogstedt. Passage through bird guts increases germination rate and seedling growth in Sorbus aucuparia. Functional Ecology, Forum Vol. 16(2002), pp.608-616.

DOI: 10.1046/j.1365-2435.2002.00668.x

[5] C.W. Taylor and W. A Gerrie. Effects of temperature on seed germination and seed. dormancy in Sorbus glabrescens Cardot. Acta Horticulturae, Forum Vol. 215(1987), pp.185-192.

DOI: 10.17660/actahortic.1987.215.25

[6] T. Yagihashi, M. Hayashida and T. Miyamoto. Effects of bird ingestion on seed germination of Sorbus commixta. Oecologia, Forum Vol. 114 (1998), pp.209-210.

DOI: 10.1007/s004420050438

[7] Y.L. Zhou. in: Arboretum of HeiLongJiang. edtied by Science and Technology Publishing of Hei Longjiang, Harbin(1986), in Press, pp.334-335.

[8] S.A. Merkle and C.J. Nairn . Hardwood tree biotechnology. Vitro Cell Dev Biol Plant, Forum Vol. 41(2005), p.602–619.

DOI: 10.1079/ivp2005687

[9] S. Lall, Z. Mandegaran and A.V. Roberts. Shoot multiplication and adventitious regeneration in Sorbus aucuparia. Plant Cell, Tissue and Organ Culture, Forum 85(2006), p.23–29.

DOI: 10.1007/s11240-005-9045-3

[10] S.W. Kim, M.J. Oh and J.R. Liu. Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose. Plant Biotechnology Report, Forum Vol. 3 (2009), p.199–203.

DOI: 10.1007/s11816-009-0092-9

[11] E. Carneros, C. Celestino, K. Klimaszewska, Y.S. Park, M. Toribio and J.M. Bonga. Plant regeneration in stone pine (Pinus pinea L. ) by somatic embryogenesis. Plant Cell Tiss Organ Cult. Forum Vol. 98 (2009), p.165–178.

DOI: 10.1007/s11240-009-9549-3

[12] Q.Y. Tang and G.M. Feng. in: Practical statistical analysis and DPS data handling system. edtied by Science Publishing, Beijing (2002), in Press, pp.156-157.