Isolation from Tannery Wastewater and Characterization of Bacterial Strain Involved in Nonionic Surfactant Degradation
A bacterial strain OPQa3 capable of utilizing nonylphenol polyoxyethylene (NPnEO) as sole carbon source was isolated from water samples collected from tannery waste treatment plant suffered long-time application of NPnEO by enrichment method. It was preliminarily identified as Brevundimonas sp. (most similar to Brevundimonas diminuta (EU434566.1)), according to the observation of morphological characteristics, physiological-biochemical tests and the similarity analysis of its 16S rDNA gene sequence. Inoculated 2% of OPQa3 suspension, to give a final OD600 of approximately 0.70, to inorganic salt medium in which the initial concentration of NPnEO was 746 mg•L-1, degradation test showed that, the degradation rate of strain OPQa3 was 84.5% within 120 hours, the optimum temperature was 30°C while the optimum pH value was about 7, and the growth period was of 24 hours.
Yanguo Shi and Jinlong Zuo
B. H. Yan et al., "Isolation from Tannery Wastewater and Characterization of Bacterial Strain Involved in Nonionic Surfactant Degradation", Advanced Materials Research, Vols. 183-185, pp. 22-26, 2011