Isolation from Tannery Wastewater and Characterization of Bacterial Strain Involved in Nonionic Surfactant Degradation

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Abstract:

A bacterial strain OPQa3 capable of utilizing nonylphenol polyoxyethylene (NPnEO) as sole carbon source was isolated from water samples collected from tannery waste treatment plant suffered long-time application of NPnEO by enrichment method. It was preliminarily identified as Brevundimonas sp. (most similar to Brevundimonas diminuta (EU434566.1)), according to the observation of morphological characteristics, physiological-biochemical tests and the similarity analysis of its 16S rDNA gene sequence. Inoculated 2% of OPQa3 suspension, to give a final OD600 of approximately 0.70, to inorganic salt medium in which the initial concentration of NPnEO was 746 mg•L-1, degradation test showed that, the degradation rate of strain OPQa3 was 84.5% within 120 hours, the optimum temperature was 30°C while the optimum pH value was about 7, and the growth period was of 24 hours.

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Advanced Materials Research (Volumes 183-185)

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22-26

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January 2011

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© 2011 Trans Tech Publications Ltd. All Rights Reserved

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