Analysis of Acidic End Products and Molecular Characterization of Biohydrogenbacterium R3 sp.nov
Biohydrogenbacterium R3 sp.nov, as a typical strains of hydrogen production bacteria, was isolated from the activated sludge in the biohydrogen production reaction by using culture media of HPB-LR which was designed particularly for isolating anaerobic fermentation bacteria and an improved Hungate rolling tubes technique and a plate method of culture bottle (PMCB) which were used for counting and isolating the anaerobes by our lab. Two representative strains of hydrogen production bacteria, namely RL20, RL37, and a strain of no hydrogen production bacterium, namely RL16 were used as contrast analysis. Experiment results indicated that the distributed proportion of ethanol and acetic acid was 95%-99% in the total amount of VAFs (Volatile Fatty Acid) of R3 which were typical ethanol-type fermentation, and the total amount of ethanol and acetic acid was commensurate with that of propionic acid.
Yanguo Shi and Jinlong Zuo
Y. T. Duan et al., "Analysis of Acidic End Products and Molecular Characterization of Biohydrogenbacterium R3 sp.nov", Advanced Materials Research, Vols. 183-185, pp. 27-30, 2011