Analysis of Acidic End Products and Molecular Characterization of Biohydrogenbacterium R3 sp.nov

Yi Tong Duan; Fang Jing Liu; Lu Wang; Yong Feng Li

doi:10.4028/www.scientific.net/AMR.183-185.27

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 183-185Analysis of Acidic End Products and Molecular...

Analysis of Acidic End Products and Molecular Characterization of Biohydrogenbacterium R3 sp.nov

Abstract:

Biohydrogenbacterium R3 sp.nov, as a typical strains of hydrogen production bacteria, was isolated from the activated sludge in the biohydrogen production reaction by using culture media of HPB-LR which was designed particularly for isolating anaerobic fermentation bacteria and an improved Hungate rolling tubes technique and a plate method of culture bottle (PMCB) which were used for counting and isolating the anaerobes by our lab. Two representative strains of hydrogen production bacteria, namely RL20, RL37, and a strain of no hydrogen production bacterium, namely RL16 were used as contrast analysis. Experiment results indicated that the distributed proportion of ethanol and acetic acid was 95%-99% in the total amount of VAFs (Volatile Fatty Acid) of R3 which were typical ethanol-type fermentation, and the total amount of ethanol and acetic acid was commensurate with that of propionic acid.

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Periodical:

Advanced Materials Research (Volumes 183-185)

Pages:

27-30

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.183-185.27

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Online since:

January 2011

Authors:

Yi Tong Duan, Fang Jing Liu, Lu Wang, Yong Feng Li

Keywords:

Biohydrogen Production, Biohydrogenbacterium R3 Sp.nov, Ethanol-Type Fermentation, Gas Chromatography (GC)

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References

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DOI: 10.1016/s0360-3199(03)00094-6

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