The Single Factor Research on the Optimal Conditions of Laccase Immobilization on the Resin
The optimal conditions of immobilization were obtained: the optimization of crosslinking agent concentration 10%, crosslinking time 2 hours、crosslinking temperature 40°C, pH 6.0, enzyme dilute multiples 500, PEB ion concentration 2.0M, immobilization time 6h and immobilization temperature 30°C, respectively.
Yanguo Shi and Jinlong Zuo
J. Zhang et al., "The Single Factor Research on the Optimal Conditions of Laccase Immobilization on the Resin", Advanced Materials Research, Vols. 183-185, pp. 2211-2215, 2011