Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli

Abstract:

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The expression and functional analysis of plant metallothionein type 2 from Brassica juncea were achieved in E. coli. The BjMT-2 cDNA was cloned into the pETM-20 vector fused to the Thioredoxin gene and induced to E. coli. Northern blot and SDS PAGE analysis showed, respectively, that the transcription of the TrxA::BjMT-2 fusion gene and the expression of the fusion protein were predominately in E. coli cells after IPTG induction. The BjMT-2 in the crude extraction prepared from the E. coli cells bearing TrxA::BjMT-2 vector were reacted with the antibody directed against BjMT-2. The E. coli cells exhibited an increased tolerance against Cu2+ and Cd2+ after BjMT-2 expression.

Info:

Periodical:

Advanced Materials Research (Volumes 183-185)

Edited by:

Yanguo Shi and Jinlong Zuo

Pages:

65-69

DOI:

10.4028/www.scientific.net/AMR.183-185.65

Citation:

Y. Z. Song et al., "Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli", Advanced Materials Research, Vols. 183-185, pp. 65-69, 2011

Online since:

January 2011

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$35.00

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