Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli

Abstract:

The expression and functional analysis of plant metallothionein type 2 from Brassica juncea were achieved in E. coli. The BjMT-2 cDNA was cloned into the pETM-20 vector fused to the Thioredoxin gene and induced to E. coli. Northern blot and SDS PAGE analysis showed, respectively, that the transcription of the TrxA::BjMT-2 fusion gene and the expression of the fusion protein were predominately in E. coli cells after IPTG induction. The BjMT-2 in the crude extraction prepared from the E. coli cells bearing TrxA::BjMT-2 vector were reacted with the antibody directed against BjMT-2. The E. coli cells exhibited an increased tolerance against Cu²⁺ and Cd²⁺ after BjMT-2 expression.

• Periodical: Advanced Materials Research (Volumes 183-185)
• Pages: 65-69
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.183-185.65
• Online since: January 2011
• Authors: Yu Zhen Song, Ping Ping Li, Ye Jie Du
• Keywords: BjMT-2, Cadmium (Cd), Copper, Escherichia coli, Expression, TrxA::BjMT-2

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