Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli
The expression and functional analysis of plant metallothionein type 2 from Brassica juncea were achieved in E. coli. The BjMT-2 cDNA was cloned into the pETM-20 vector fused to the Thioredoxin gene and induced to E. coli. Northern blot and SDS PAGE analysis showed, respectively, that the transcription of the TrxA::BjMT-2 fusion gene and the expression of the fusion protein were predominately in E. coli cells after IPTG induction. The BjMT-2 in the crude extraction prepared from the E. coli cells bearing TrxA::BjMT-2 vector were reacted with the antibody directed against BjMT-2. The E. coli cells exhibited an increased tolerance against Cu2+ and Cd2+ after BjMT-2 expression.
Yanguo Shi and Jinlong Zuo
Y. Z. Song et al., "Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli", Advanced Materials Research, Vols. 183-185, pp. 65-69, 2011