Isolation and Characterization of Laccase Activity in a Novel Bacillus amyloliquefaciens LC02

Abstract:

Bacterial strains exhibiting laccase activity were isolated from the forest soil. A strain LC02 with syringaldazine oxidation ability was obtained using enrichment medium supplemented with copper ions. The isolated strain was identified as Bacillus amyloliquefaciens using physiological and biochemical tests as well as 16S rDNA sequence analysis. The characterization of spore laccase activity was investigated. The result showed that the optimum pH and temperature of the enzyme was 6.6 and 70°C, respectively. A great thermostability was observed for the spore laccase at 70°C. Laccase activity was strongly inhibited by 0.1 mmol/L NaN3, dithiothreitol and cysteine.

• Periodical: Advanced Materials Research (Volumes 183-185)
• Pages: 773-777
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.183-185.773
• Online since: January 2011
• Authors: Jun Bo Pan, Min Zhao, Lei Lu, Mei Hui Du, Guo Fu Li, Jun Li, Tian Nv Wang, Xiao Liang Tang
• Keywords: Bacillus amyloliquefaciens, Characterization, Isolation, Laccase

References

[1] A.M. Mayer and R.C. Staples: Phytochemistry Vol. 60(2002), p.551–565.

[2] P. Sharma, R. Goel and N. Capalash: World J. Microbiol. Biotechnol. Vol. 23(2007), p.823–832.

[3] S.R. Couto and J.L.T. Herrera: Biotechnol. Adv. Vol. 24(2006), p.500–513.

[4] X.Z. Dong and M.Y. Cai: Manual of systematic identification for common bacteria (Science Press, Beijing 2001).

[5] M. Wang, J.K. Chen and B. Li: Pedosph. Vol. 17 (2007), p.545−556.

[6] R.C. Goldman and D.J. Tipper: J. Bacteriol. Vol. 135 (1978), p.1091–1106.

[7] L.O. Martins, C.M. Soares, M.M. Pereira, M. Teixeira, T. Costa, G.H. Jones and A.O. Henriques: J. Biol. Chem. Vol. 277 (2002), p.18849–18859.

[8] P. Baldrian: FEMS Microbiol. Rev. Vol. 30 (2006), p.215–242.

[9] G. Diamantidis, A. Effosse, P. Potier and R. Bally: Soil Biol. Biochem. Vol. 32(2000), p.919–927.