This study proposes to develop a best treatment interface of motion trajectory as a construct for children to build up their sense of spatiality. There are 30 children from kindergarten participate in this research. There are two factors in the study, first is the “leading object” and the other is the “reference object”, and each factor contains two levels, hence, there are four treatments in this experiment. This research uses “maze” as a spatial sense conceptual tool, and the maze is constructed by 3D models as a virtual environment. The focus of this research is to find out the best treatment and does not cause cognitive burden on children in the meantime. The result of this research is that the best tr eatment of motion trajectory depends on the treatment of “leading object” with “reference object” for children when they grab spatial concepts.