Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage

Yan Fei Zhang; Yu Yang; Jian She Liu; Guan Zhou Qiu

doi:10.4028/www.scientific.net/AMR.20-21.473

Paper Titles

Quantification of Biomining Microorganisms Using Quantitative Real-Time Polymerase Chain Reaction
p.457

Chalcopyrite and Bornite Differentially Affect Protein Synthesis in Planktonic Cells of Acidithiobacillus ferrooxidans
p.461

Temperature Effects on the Iron Oxidation Kinetics of a Leptospirillum ferriphilum Dominated Culture at pH Below One
p.465

Metabolism Peculiarities of Bacteria of the Genus Sulfobacillus
p.469

Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage
p.473

Bacterial Oxidation of Elemental Sulfur: Changes in Oxidation Kinetics
p.477

Quantification of Microorganisms Involved in Cemented Layer Formation in Sulfidic Mine Waste Tailings (Freiberg, Saxony, Germany)
p.481

Analysis of the Microbial Community in the Leachate Collected from an Experimental Bioleaching Column by Cloning and RFLP
p.485

The Diversity of Benthic Microorganisms in Acidic Mine Lake Sediments
p.489

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Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage

Abstract:

The acidophilic heterotrophic bacteria Acidiphilium spp. were considered as a ministrant of Leptospirillum spp., Acidithiobacillus spp. and other autotrophic bacteria in acid mine drainage (AMD). 25 Acidiphilium-like bacterial strains were isolated from eight different mines in China and their physiology was characterized. Their marmatite-bioleaching ability has been investigated and compared with that of a mixed culture of Acidiphilium sp. and Acidithiobacillus ferrooxidans. The results indicated that some Acidiphilium-like strains exhibited a high marmatite-bioleaching ability, which was even higher than that of Acidithiobacillus ferrooxidans. The leaching efficiency was not higher in mixed than in pure culture. The phylogenetic relationship of the 25 Acidiphilium-like strains was analyzed by 16S rRNA sequencing, GyrB sequencing and rep-PCR genomic fingerprinting. The 25 strains and other eight species of Acidiphilium spp. were clustered into three groups.