Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage

Abstract:

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The acidophilic heterotrophic bacteria Acidiphilium spp. were considered as a ministrant of Leptospirillum spp., Acidithiobacillus spp. and other autotrophic bacteria in acid mine drainage (AMD). 25 Acidiphilium-like bacterial strains were isolated from eight different mines in China and their physiology was characterized. Their marmatite-bioleaching ability has been investigated and compared with that of a mixed culture of Acidiphilium sp. and Acidithiobacillus ferrooxidans. The results indicated that some Acidiphilium-like strains exhibited a high marmatite-bioleaching ability, which was even higher than that of Acidithiobacillus ferrooxidans. The leaching efficiency was not higher in mixed than in pure culture. The phylogenetic relationship of the 25 Acidiphilium-like strains was analyzed by 16S rRNA sequencing, GyrB sequencing and rep-PCR genomic fingerprinting. The 25 strains and other eight species of Acidiphilium spp. were clustered into three groups.

Info:

Periodical:

Advanced Materials Research (Volumes 20-21)

Edited by:

Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher

Pages:

473-476

DOI:

10.4028/www.scientific.net/AMR.20-21.473

Citation:

Y. F. Zhang et al., "Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage", Advanced Materials Research, Vols. 20-21, pp. 473-476, 2007

Online since:

July 2007

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$35.00

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