Heavy Metal Tolerance and Copper Uptake in Yeast Isolated from Patagonia/ Argentina

Article Preview

Abstract:

In recent years the use of microbial methods for decontamination or recovery of heavy metals from environment has increased. Microorganisms such as yeasts are potential bioremediators, removing metals via active or passive uptake. Pink-coloured and pigment-less yeast strains isolated from Agrio River, Patagonia Argentina, were tested for copper, nickel, cadmium and zinc tolerance. An agar-plate qualitative screening method using YNB-glucose agar at different metal concentrations was employed. The tolerance to the metals varied depending on the strain tested. A pigmented yeast strain (Agrio 16) was selected by its tolerance. The ability of this strain to copper uptake was investigated. The kinetics of bioaccumulation/biosorption with increasing copper concentrations up to 622.8 mg l-1 were carried out using viable and nonviable biomass. The values of constants k and n obtained for the Freundlich model are 0.0418 and 0.7430, respectively. The maximun sorption uptake capacity (q) for viable biomass was 81.6 mg of copper/g of biomass.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 20-21)

Pages:

639-642

Citation:

Online since:

July 2007

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2007 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] B. Volesky: in Sorption and Biosorption, Chapter 4, publ. BV Sorbex, Inc. Canada (2003).

Google Scholar

[2] K. J. Blackwell, I. Singleton and J. M. Tobin: Appl. Microbiol. Biotehnol. Vol. 43 (1995), p.579.

Google Scholar

[3] D. Brady and J. R. Duncan: Appl. Microbiol. Biotehnol. Vol. 41 (1994), p.149.

Google Scholar

[4] G. Dönmez and Z. Aksu: Process Biochem. Vol. 35 (1999), p.135.

Google Scholar

[5] P. Kaszycki, D. Fedorovych, H. Ksheminska, L. Babyak, D. Wojcik and H. Koloczek: Microb. Res. Vol. 159 (2004), p.11.

DOI: 10.1016/j.micres.2003.12.002

Google Scholar

[6] L. Villegas, M. J. Amoroso and L. I. C de Figueroa, in: Methods in Biotechnology: Environmental Microbiology: Methods and Protocols, edited by J.F.T. Spencer and L. Ragout de Spencer, Chapter 24, Humana Press Inc. (2004), p.249.

Google Scholar

[20] [40] [60] [80] 100 120 140 160 180 0 100 200 300 400 500 600 Cf (mg l -1) q (mg g -1 ) y = 1. 3459x - 3. 1745 R.

Google Scholar

[2] = 0. 9733.

Google Scholar

[1] [2] [3] [4] [5] [6] 01234567 ln Cf ln q A B.

Google Scholar

0. 2 0. 4 0. 6 0. 8.

Google Scholar

[1] 1. 2 2. 7 2. 5 4. 4 7. 9 11. 9 50 81. 6 q (mg g-1) X/X control 49. 2% 29. 3% 27. 8% 23. 8% 18. 1% 18. 3% 11. 7.

Google Scholar