Portable Benzo[a]pyrene Immunosensor Based on the Polyanline Nanofiber Modified Screen-Printed Electrode

Abstract:

In this work, polyaniline nanofiber was synthesized. Then, polyaniline nanofiber and chitosan were dropped onto the surface of the self-prepared screen-printed electrode (SPE), respectively. After aminopyrene-BSA conjugate was immobilized on the modified electrode surface with the aid of EDC/NHS solution, the primary antibody was dropped on the modified SPE and followed by the HRP-labeled secondary antibody, thus a disposable immunosensor was developed for the rapid detection of benzo[a]pyrene based on an indirect immunoassay.

• Periodical: Advanced Materials Research (Volume 221)
• Pages: 295-301
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.221.295
• Online since: March 2011
• Authors: Chun Ping Wang, Jie Dan Zhou, Yi Bin Huang, Gen Quan Liang, Ying Ju Liu, Hong Tao Lei
• Keywords: Benzo[a]pyrene, Immunosensor, Nanofiber, Screen-Printed Electrode

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