Expression of Trichoderma reesei β-Xylanase in Escherichia coli and its Function in Degradation of Juncao Holocellulose

Li Hua Liu; Zhi Wei Lin; Ling Lin; Yan Ling Yang; Zhan Xi Lin; Shi Hua Wang

doi:10.4028/www.scientific.net/AMR.236-238.1058

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 236-238Expression of Trichoderma reesei β-Xylanase in...

Expression of Trichoderma reesei β-Xylanase in Escherichia coli and its Function in Degradation of Juncao Holocellulose

Abstract:

In this study, the xyn2 gene, which encodes an endo-β-1,4-xylanase, was isolated with holocellulose extracted from Juncao Miscanthus floridulu as an inducer. The xyn2 gene expressed in Escherichia coli, with the estimated yield of 349 U·mL^-1. Zymogram analysis showed that the purified Xyn2 had only one band on SDS-PAGE with an estimated molecular mass of 28 kDa. Enzymology analysis demonstrated that its optimum activity was at pH 6.0 and 60°C, with stability at pH range 4.0~7.0 and temperature up to 50°C. The metal ions Cu²⁺ and Mg²⁺ showed some inhibition effects, while Fe²⁺ and Fe³⁺ had small stimulating effects. Its values of Km and Vmax are 2.85 mM and 50.2 mM/min, respectively. Based on our results, we propose a novel way to convert Juncao biomass into energy and other useful products.

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Periodical:

Advanced Materials Research (Volumes 236-238)

Pages:

1058-1062

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.236-238.1058

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Online since:

May 2011

Authors:

Li Hua Liu, Zhi Wei Lin, Ling Lin, Yan Ling Yang, Zhan Xi Lin, Shi Hua Wang

Keywords:

Holocellulose Degradation, Juncao Biomass, Trichoderma reesei, Xylanase

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