Inducement Expression and Synthetic Dyes Decolourization Capacity of CotA

Ning Zhang; Min Zhao; Chun Lei Wang; Guang Ying Hui Du

doi:10.4028/www.scientific.net/AMR.236-238.2344

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 236-238Inducement Expression and Synthetic Dyes...

Inducement Expression and Synthetic Dyes Decolourization Capacity of CotA

Abstract:

Bacillus subtilis cotA gene which was designed According to GenBank. cotA gene was transformed to Escherichia coli BL21(DE3). The recombined CotA protein was expressed by IPTG induced method. The CotA protein was stained red by syringaldazine after Native-PAGE.The effects of the condition of induction expression on enzyme production of recombinant Escherichia coli were investigated. The result showed that the highest enzyme activity of recombinant CotA could be achieved under the following conditions: the concentration of IPTG was 1.0 mmol L^-1, IPTG was added to the culture given a final concentration of 1.0 mol L^-1 when the OD₆₀₀ of culture reached 1.0, induction time was 12 h at 25°C. The CotA laccases could decolorize Remazol brilliant blue R (RBBR) and Congo red with 87% and Isatin and Crystal Violet with 55%. The result indicated that the CotA laccase had the potential to be industrial enzyme.

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Periodical:

Advanced Materials Research (Volumes 236-238)

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2344-2348

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.236-238.2344

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Online since:

May 2011

Authors:

Ning Zhang, Min Zhao, Chun Lei Wang, Guang Ying Hui Du

Keywords:

Decolorization, Induction Optimization, Recombinant Bacterial Laccase

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