Ultrasonic-Assisted Preparation, Characterization and Antibacterial Activity of β-Chitosan from Squid Pens

Abstract:

β-chitosan preparation from squid pens was carried out using aqueous NaOH with the ultrasonic assistance. Single factor experiments and L₉ (3⁴) orthogonal experiments were used to investigate the effect of three parameters (reaction time, concentration of NaOH and reaction temperature) on deacetylation of β-chitin. The optimal conditions for deacetylation of chitin were reaction temperature 80°C, reaction time 2 h and concentration of NaOH 50%. The optimal conditions allowed deacetylation degree of β-chitin from 71.32% to 92.91%. The β-chitosan from squid pens was confirmed by Fourier transform infrared spectroscopy. The antibacterial activities of the prepared β-chitosans again staphylococcus aureus (S. aureus) and Escherchia coli (E.coli) were then determined and compared by the MIC (minimum inhibitory concentration). Results indicate that β-chitosans with different degrees of deacetylation (DD) possess different antibacterial activity. The growth of S. aureus can be easily inhibited by prepared β-chitosan than E.coli.

• Periodical: Advanced Materials Research (Volumes 236-238)
• Pages: 282-287
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.236-238.282
• Online since: May 2011
• Authors: Jun Huang, Jian Wei Mao, Sheng Hu, Dong Ke Zhao, Le He Mei, Shi Wang Liu, Yuan Feng Wu, Sheng Fang, Qian Shao
• Keywords: Antibacterial Activity, Deacetylation, Ultrasonic Assistance, β-Chitin, β-Chitosan

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