Enzymatic hydrolysis constitutes an attractive process configuration for ethanol production from biomass. However, the high enzyme addition contributes to a high process cost. This study shows that appropriate concentration of Gleditsia saponin could reduce the enzyme loading and increase β-glucosidase active. The optimal conditions of enzymatic hydrolysis process of furfural residues was at enzyme loading of 6 FPU cellulose/g substrate and 9 IU β-glucosidase/g substrate with 0.6 g/l of Gleditsia saponin. The enzyme loading could be reduced by 50% with addition of 0.64 g/l Gleditsia saponin during furfural residues hydrolysis. The β-glucosidase activity from hydrolysate with 0.64 g/l Gleditsia saponin was higher than that without any surfactants.