Immobilization of Glucose Oxidase on L-lysine Modifided Chitosan

Abstract:

A L-lysine modified semi-crosslinked chitosan (LMCCT) beads was synthesized and demonstrated to be an excellent enzyme support by studying the enzymatic properties of glucose oxidases (GODs) immobilized on it. The prepared LMCCT beads have very larger BET specific surface ( 403 m²/g) and excellent chemical stability due to the semi-crosslinking of chitosan backbones. The insertion of the flexible L-lysine spacers between chitosan backbone and the active amino groups increases the activity of immobilized GODs (I-GODs) and improves the affinity of I-GODs towards the substrate, which was testified by the smaller Michaealis constant, K_m and larger reactive rate, V_m of I-GODs on LMCCT than that on crosslinked chitosan. Further studies of I-GOD activity at varied temperature and pH also indicated that I-GOD on LMCCT has an excellent stability.

• Periodical: Advanced Materials Research (Volumes 236-238)
• Pages: 909-912
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.236-238.909
• Online since: May 2011
• Authors: Yu Ting Xu, Li Chun Dong, Da Fu Huang, Yan Xiao, Xiao Hua Zhou
• Keywords: Chitosan (CS), Enzyme Immobilization, Enzyme Support, L-Lysine, L-Lysine Modified Crosslinked Chitosan

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