Immobilization of Glucose Oxidase on L-lysine Modifided Chitosan
A L-lysine modified semi-crosslinked chitosan (LMCCT) beads was synthesized and demonstrated to be an excellent enzyme support by studying the enzymatic properties of glucose oxidases (GODs) immobilized on it. The prepared LMCCT beads have very larger BET specific surface ( 403 m2/g) and excellent chemical stability due to the semi-crosslinking of chitosan backbones. The insertion of the flexible L-lysine spacers between chitosan backbone and the active amino groups increases the activity of immobilized GODs (I-GODs) and improves the affinity of I-GODs towards the substrate, which was testified by the smaller Michaealis constant, Km and larger reactive rate, Vm of I-GODs on LMCCT than that on crosslinked chitosan. Further studies of I-GOD activity at varied temperature and pH also indicated that I-GOD on LMCCT has an excellent stability.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
Y. T. Xu et al., "Immobilization of Glucose Oxidase on L-lysine Modifided Chitosan", Advanced Materials Research, Vols. 236-238, pp. 909-912, 2011