Optimal Study of Co-Immobilized GOD/CAT on Cross-Linked Chitosan Microsphere Containing L-Lysine

Abstract:

Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on the cross-linked chitosan microsphere containing L-Lysine (CCL) by the method of absorption-crosslink, with cross-linking agent of glutaraldehyde. The optimized conditions of the co-immobilization are as follows: activity ratio of GOD and CAT is 0.78:1, concentration of glutaraldehyde is 0.17mmol/L, and the immobilizing process last for 3 hours. The enzyme activity of co-immobilized enzyme is 15.3U/mg and activity recovery is 50.1%. The half life is 231 days, 2.4 times of immobilized GOD.

• Periodical: Advanced Materials Research (Volumes 236-238)
• Pages: 926-929
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.236-238.926
• Online since: May 2011
• Authors: Xiao Hua Zhou, Ying Li Wang, Xia Li Su
• Keywords: Co-Immobilized GOD/CAT, Cross-Linked Chitosan, L-Lysine, Optimal

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