Optimal Study of Co-Immobilized GOD/CAT on Cross-Linked Chitosan Microsphere Containing L-Lysine
Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on the cross-linked chitosan microsphere containing L-Lysine (CCL) by the method of absorption-crosslink, with cross-linking agent of glutaraldehyde. The optimized conditions of the co-immobilization are as follows: activity ratio of GOD and CAT is 0.78:1, concentration of glutaraldehyde is 0.17mmol/L, and the immobilizing process last for 3 hours. The enzyme activity of co-immobilized enzyme is 15.3U/mg and activity recovery is 50.1%. The half life is 231 days, 2.4 times of immobilized GOD.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
X. H. Zhou et al., "Optimal Study of Co-Immobilized GOD/CAT on Cross-Linked Chitosan Microsphere Containing L-Lysine", Advanced Materials Research, Vols. 236-238, pp. 926-929, 2011