The ATRP of N-isopropylacrylamide (NIPAM) was carried out in DMF/water at 60 °C to afford a novel linear end-functionalized poly(N-isopropylacrylamide) with acetylated β-CD (AcCD-PNIPAM) and the structures of the products were characterized. The number-average molecular weight (Mn) and the polydispersity index (PDI) of the polymer were obtained from gel permeation chromatography (GPC) analysis. The end-functionalized PNIPAM with β-CD (CD-PNIPAM) was obtained by deacetylation from AcCD-PNIPAM. The lower critical solution temperature (LCST) for the aqueous solutions of CD-PNIPAM, measured by means of ultraviolet-visible spectroscopy (UV-vis), was 4~5 °C higher than that of AcCD-PNIPAM. Ferrocene was included into CD-PNIPAM with mole ratio of 1:1 and the inclusion complex was characterized by cyclic voltammetry (CV) technology.