The behaviour of composite cellular floor beams is becoming important as such members are increasingly used in multistory buildings. But, in the design of some special cellular beam with sharp corner web holes such as eye-shaped openings, the issue of low cycle fatigue failure becomes increasingly critical. In this paper, three-dimensional nonlinear finite element models of composite cellular floor beams with eye-shaped openings have been developed, taking into consideration the influence of the changes in degree of shear connections. The assessment based on stress amplitude for risk of fatigue failure under low cycle service load is presented as design guides in this paper. It is concluded that the risk grows along with the increase of composite actions in beams.