Seven groups, total of 31 single-bolted steel-glulam-steel joints, were tested for their mechanical performance. The mechanical properties of single-bolted steel-glulam-steel connection subjected to a load parallel to the grain have been studied. The failure mode and failure mechanism of bolted connections were discussed in detail. It is shown that the failure mode, bearing capacity, stiffness and ductility of the joint are mainly relative to the thickness of the glulam and the diameter of the bolt. Due to the wide application of bolted steel-glulam-steel connections in engineering, the present work can be taken as a reference in manufacture, and design of modern timber structures.