Stochastic resonance (SR) is a nonlinear phenomenon which can be used to detect weak signal. The theory of SR in a biased mono-stable system driven by multiplicative and additive white noise as well as a weak periodic signal is investigated. The virtual instrument (VI) for weak signal detecting based on this theory is designed with LabVIEW. This instrument can be used to detect weak periodic signals which meets the conditions given and can greatly improved the power spectrum of the weak signal. The results that related to different sets of parameters are given and the features of these results are in accordance with the theory of mono-stable SR. Thus, the application of this theory in the detecting of weak signal is proven to be valid.