Design the formula of UV waterless offset ink, through changing the content of padding, different ink samples that with different rheological properties were made. Proof with the IGT printability tester, and the printing qualities of inks were tested. The influence of rheological properties on the printing quality of ink was analysis. The results show that the rheological properties influence the printing quality directly. The higher the viscosity of ink is, the bigger the thixotropy is, and the smaller the fluidity is. The solid density, gloss, ink-transfer rates and dot gain of the printed matter decrease with the increase of ink’s yield value.