Anti-Inflammatory Property of Biomaterial Carotenoids Production by Rhodobacter Sphaeroides WL-APD911

Abstract:

A Rhodobacter sphaeroides WL-APD911 with the capability of producing the end products of neurosporene and ξ-carotene, rather than the normal end products of spheroidene, was isolated from hundreds of strains by using chemical mutagenesis. The strain WL-APD911 grew well in LB medium, and the colored carotenoids were produced within 3 days of incubation at 30°C. The Rs-M of colored carotenoid extracts obtained from strain WL-APD911 by using methanol extraction inhibited the NO production and iNOS expression by LPS-induced RAW 264.7 cells. This probability of reason for the Rs-M as the best source was to prompt the anti-inflammation and anti-oxidation in nutraceutical products. Finally, the carotenoid extracts (Rs-M) with neurosporene and ξ-carotene was called lycogene to be a newly biomaterial.

• Periodical: Advanced Materials Research (Volumes 287-290)
• Pages: 2028-2031
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.287-290.2028
• Online since: July 2011
• Authors: Wen Tung Wu, Wen Sheng Liu
• Keywords: Anti-Inflammation, Carotenoid, Neurosporene, Rhodobacter sphaeroides WL-APD911

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[6] M.M. Rafi, P.N. Yadav, M. Reyes, Lycopene inhibits LPS-induced proinflammatory mediator inducible nitric oxide synthase in mouse macrophage cells. J of Food Science. 72 (2007) 69-74. Fig. 1 HPLC elution profile of lycopene standard and Rs-M produced in R. sphaeroides WL-APD911. An arrow indicated the retention time of lycopene standard. Fig. 2 Profile of characteristic of the Rs-M. A and B denoted neurosporene andξ-carotene, respectively. Fig. 3 Effect of Rs-M on NO production. RAW 264.7 macrophage cells were treated with LPS (100 ng/mL) and various concentration of Rs-M with lycogene mixtures for 24 h. The amount of nitrite in supernatant from each treatment group was measured by using Griess reagent. Fig. 4. Effect of Rs-M on inhibition of LPS-induced iNOS protein expression. RAW 264.7 macrophage cells were treated with LPS (100 ng/mL) and various concentration of Rs-M with lycogene mixtures for 24 h. The detection of β-action was done in the same blot as a loading control.