Bioactivity of TiO₂/Ti Composite Membrane with Different Crystral Phase

Abstract:

The bioactivity of TiO₂/Ti composite membrane with different crystral phase was studied by the culture of hypothalamic neurons in vitro for the application of immunoisolation. The kind of membrane supported on porous Ti has been prepared by sol-gel technique and the crystral phase was controlled by different sintering temperature. The crystral phase of TiO₂, morphology and function of hypothalamic neurons were observed by X-ray diffraction (XRD), enzyme-linked immunosorbent assay (ELISA) and environmental sanning electron microscope (ESEM). The results showed that neurons could attach well to the TiO₂ /Ti composite membranes with rutile phase and became bipolar. The long axon and dendrite with dendrite spine were interconnected by synapses. A complicated neural net were easily observed. The secretory level of β-endorphine remained between 51.3pmol/L and 40.6pmol/L all the times. However, the neurons in membranes with anatase were transformed into an umbilicate structure without processes. The secretory function of neurons also died out in 4 days. These results indicated that the TiO₂ /Ti composite membranes with rutile phase were propitious to the neuron’s growth than the composite membranes with anatase phase and could be used as immunoisolation membranes.

• Periodical: Advanced Materials Research (Volumes 287-290)
• Pages: 69-72
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.287-290.69
• Online since: July 2011
• Authors: Min Jing Zhan, Gang Li, Qiang Wei, Hua Lei Cui, Ling Lin
• Keywords: Bioactivity, Encapsulation, Immunoisolation, Sol-Gel Method

References

[1] Emerich, D.F. and S.R. Winn, Immunoisolation cell therapy for CNS diseases. Crit Rev Ther Drug Carrier Syst, 2001. 18(3): 265-98.

DOI: 10.1615/critrevtherdrugcarriersyst.v18.i3.10

[2] Risbud, M.V. and R.R. Bhonde, Islet immunoisolation: experience with biopolymers. J Biomater Sci Polym Ed, 2001. 12(11): 1243-52.

DOI: 10.1163/156856201753395770

[3] Wilson, J.T. and E.L. Chaikof, Challenges and emerging technologies in the immunoisolation of cells and tissues. Adv Drug Deliv Rev, 2008. 60(2): 124-45.

DOI: 10.1016/j.addr.2007.08.034

[4] Day, J.G., et al., The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections. Cryo Letters, 2007. 28(5): 359-76.

[5] Haque, T., et al., Investigation of a new microcapsule membrane combining alginate, chitosan, polyethylene glycol and poly-L-lysine for cell transplantation applications. Int J Artif Organs, 2005. 28(6): 631-7.

DOI: 10.1177/039139880502800612

[6] Orive, G., et al., Development and optimisation of alginate-PMCG-alginate microcapsules for cell immobilisation. Int J Pharm, 2003. 259(1-2): 57-68.

DOI: 10.1016/s0378-5173(03)00201-1

[7] La Flamme, K.E., et al., Nanoporous alumina capsules for cellular macroencapsulation: transport and biocompatibility. Diabetes Technol Ther, 2005. 7(5): 684-94.

DOI: 10.1089/dia.2005.7.684

[8] Desai, T.A., D. Hansford, and M. Ferrari, Characterization of micromachined silicon membranes for immunoisolation and bioseparation applications. Journal of Membrane Science, 1999. 159(1-2): 221-231.

DOI: 10.1016/s0376-7388(99)00062-9

[9] Leoni, L. and T.A. Desai. Biotranport and biocompatibility of nanoporous biocapsules. in Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000.

DOI: 10.1109/mmb.2000.893753

[10] Hikosaka, T., et al., Application of sintered Ti powder to dental prostheses. Nihon Hotetsu Shika Gakkai Zasshi, 2005. 49(2): 242-52.

DOI: 10.2186/jjps.49.242

[11] Zou, C., et al., Preparation, microstructure and mechanical properties of porous titanium sintered by Ti fibres. J Mater Sci Mater Med, 2008. 19(1): 401-5.

DOI: 10.1007/s10856-006-0103-0

[12] De Medeiros, W.S., et al., Bioactive porous titanium: an alternative to surgical implants. Artif Organs, 2008. 32(4): 277-82.