The paper introduces the working principle of the sequential turbo-charging (STC) system of multi-turbocharger. To improve low-load performance and operating economy of the 234V12 diesel engine, a STC system of multi-turbocharger for the diesel engine was designed. Theoretical calculation and experimental research was done on this improved marine diesel engine. Then, a 3-phase STC system is presented by analyzing and comparing the test result and the switching boundary of this system is confirmed. The test results show that the low-load performance is improved obviously by use of multi-turbocharger STC system.