Construction of a New Genetic Engineering Bacterium for Preparation of Superoxide Dismutase with High Productivity

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A new Mn-SOD gene encoding 202 amino acids was cloned from genomic DNA of Bacillus subtilis ATCC 9372 for construction of a genetic engineering bacterium to produce SOD. Its phylogenetic relationships with other Bacillus spp. revealed that this predicted protein is most closely related to B. atrophaeus NRS-213 (AY197616) and B. subtilis 168. This gene was inserted into expression plasmid pET28a and first successfully expressed in E. coli BL21. The SOD was expressed accounted for approximately 45.6% of total bacterial protein. The activity of the SOD was 2553.211 U/mg, the enzyme showed maximum activity at about pH 8.0 and relatively stable from pH 6.0 to 11.0. This SOD had a good thermal stability with >75% retaining of the relative enzymatic activity after incubation at 50 °C for 90 min. This study demonstrated that a new genetic engineering bacterium to produce SOD with high productivity has been successfully constructed.

Info:

Periodical:

Advanced Materials Research (Volumes 314-316)

Edited by:

Jian Gao

Pages:

1973-1976

DOI:

10.4028/www.scientific.net/AMR.314-316.1973

Citation:

Y. Zhao et al., "Construction of a New Genetic Engineering Bacterium for Preparation of Superoxide Dismutase with High Productivity", Advanced Materials Research, Vols. 314-316, pp. 1973-1976, 2011

Online since:

August 2011

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$35.00

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