Measurement of Diffuse Reflectance Spectrum of Biological Material with Single Integrating-Sphere

Abstract:

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Detection of interactions between light and biological material can be used to characterize the optical properties of the biological material. The purpose of this paper is to develop a method that measures the diffuse reflectance spectrum of biological material with single integrating-sphere. The experimental system incorporated a DH-2000 Deuterium Tungsten Halogen Light Sources, USB4000-VIS-NIR Miniature Fiber Optic Spectrometer and an integrating-sphere. Intralipid and ink were used to mimic scattering and absorbing properties of biological material in the tested sample. Total of 30 sample representing of normal and malignant human breast tissue were measured. The measured optical reflectance spectrums with different scattering and absorbing properties were analyzed. The result showed that the diffuse reflectance spectrums of normal and malignant biological material are different in intensity.

Info:

Periodical:

Edited by:

Helen Zhang and David Jin

Pages:

252-255

DOI:

10.4028/www.scientific.net/AMR.321.252

Citation:

L. S. Zhang et al., "Measurement of Diffuse Reflectance Spectrum of Biological Material with Single Integrating-Sphere", Advanced Materials Research, Vol. 321, pp. 252-255, 2011

Online since:

August 2011

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$35.00

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