To study the influence of negative oxygen ions fiber content on the released negative oxygen ions concentration of the fabric and the effect of different organizational structures on wearability of negative oxygen ions fabrics, the blended yarns of modified polyester negative oxygen ions fiber and viscose fiber in different blending ratio were selected, two series of fabrics were manufactured for the experiment. The released amount of negative oxygen ions and wearability of the blended fabrics were tested and analyzed in this paper. The results showed that with the increasing of negative oxygen ions fiber content in fabrics, the released negative oxygen ions concentration had increased. When the blended ratio of negative oxygen ions fiber and viscose fiber in fabric was 50/50, the released negative oxygen ions concentration of the fabric reached up to 4810/cm3 and was close to that of the field. Wearability of honeycomb weave fabric was best and suitable for home textiles through comprehensive analysis, followed by satin fabric. The conclusion from this research can be used as a reference to design and develop negative oxygen ions home textiles.