In the present paper, the influences of fiber hygroscopicity on elastic knitted cotton and cotton blended Modal (50/50) fabric in heat and moisture transfer properties and comfort were studied. The rate of moisture regain, moisture transportation and liquid wicking performance were tested. Besides, wear trials were carried to collect subjective evaluation of thermo-moisture comfort sensation of the wearer. It is found that elastic knitted fabric of cotton-Modal/Spandex has better water vapour absorption and permeability as well as better liquid water transfer and spreading capacity. During wear trial, cotton-Modal/spandex fabric B caused significantly lower wet and sticking perception than cotton/spandex fabric A.